Agricultural and Biological Sciences Journal
Articles Information
Agricultural and Biological Sciences Journal, Vol.1, No.2, Apr. 2015, Pub. Date: Mar. 21, 2015
Genetic Characterization of Lentil (Lens culinaris L.) Germplasm by Using SSR Markers
Pages: 16-26 Views: 2933 Downloads: 1761
[01] U. K. S. Kushwaha, Institute of Agriculture and Animal Science, Department of Plant Breeding, Tribhuvan University, Rampur, Chitwan, Nepal.
[02] S. K. Ghimire, Institute of Agriculture and Animal Science, Department of Plant Breeding, Tribhuvan University, Rampur, Chitwan, Nepal.
[03] N. K. Yadav, Nepal Agricultural Research Council, Singh Darbar, Kathmandu, Nepal.
[04] B. R. Ojha, Institute of Agriculture and Animal Science, Department of Plant Breeding, Tribhuvan University, Rampur, Chitwan, Nepal.
[05] R. K. Niroula, Nepal Agricultural Research Council, Biotechnology Unit, Khumaltar, Lalitpur.
Ninety six lentil accessions were collected from National Grain Legume Research Program, Rampur; Regional Agriculture Research Station, Nepalgunj and National Agriculture Genetic Resource Center, Khumaltar, Lalitpur.Among them; four lines were Nepal Local, forty two lines were Nepal Cross; forty seven lines were ICARDA Line and finally three lines were Indian Line. Allaccessions were analysed by DNA fingerprinting using thirty three selected polymorphic SSR markers. The characterization was performed in Biotechnology Unit, Nepal Agricultural Research Council, Khumaltar, Lalitpurin 2012 by using standard protocols. The dendrogram constructed based on Jaccard’s similarity coefficient and UPGMA separated all accessions into four clusters with two major groups. Group A consisted of three cluster (I, II and III) whereas Group B comprised only one cluster (IV). Cluster I represented 58.33 % of total genotypes followed by cluster II (38.54%), cluster III (2.08 %) and cluster IV (1.04 %). Cluster I was the largest and cluster IV was the smallest cluster based on number of genotypes. Microsatellite profiling based dendrogram revealed that RL lines were more diverged than ILL lines. Among all lentil accessions Sagun and LN 0135 showed distant relationship as compared to others in dendrogram. The first three Eigen values of three principal coordinate axes explained 71.08 % genetic variation and the first two principal axes accounted for 53.76 % of total variation describing ninety six genotypes. The clustering pattern obtained coincided with the apparent grouping patterns performed by PCA. So the results obtained through PCA were confirmed by non-hierarchical clustering. All the accessions included in the study displayed significant amount of genetic variability due to different center of origin and different genetic constitutions. The diversity detected in this study may constitute the new materials for future systematic lentil breeding programs.
Lentil, Genotypes, Diversity, Cluster, Marker
[01] Ahmad, M., D.L. McNeil and A.G. Fautrier. 1997. Phylogenetic relationships inLensspecies and parentage determination of their interspecifichybrids using RAPD markers. Euphytica 94: 101-110.
[02] Alabboud, I., L. Szilagyi and G.H.V. Roman. 2009. Assessment of genetic diversity in lentil (Lens culinaris Medik) as revealed by RAPD markers. Scientific papers, USAMV Bucharest, Series A, Vol. LII. 439p.
[03] Anonymous. 2003. Lentil: situation and outlook. Bi-weekly Bulletin,13(21)Available at: (Retrieved on 23rd December 2012).
[04] Bahl, P.N., S. Lal and B. M. Sharma. 1993.An overview of the production and problems inSoutheast Asia. In W. Erskine and M. C. Saxena (eds.)Lentil in South Asia. Proceedings of theseminar on lentils in south Asia ICARDA, Aleppo, Syria. pp.1-10.
[05] Bhatty, R.S. 1998. Composition and quality of lentil (Lens culinarisMedik): A review. Canadian Institute of FoodScience and Technology 21: 144–160.
[06] Chakraborty, M. and M.F. Haque. 2000. Genetic variability and component analysis in lentil (Lens culinaris Medik). J Res.Birsa Agric. Uni. 12(2): 199-204.
[07] Cubero, J.I. 1981. Origin, taxonomy and domestication. In: Webb C,Hawtin G (Eds.), Lentils, pp: 15-38. C.A.B., Landon, UK.
[08] Doyle, J.J. and J.L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11-15.
[09] Erskine, W., B. Bayaa and M.C. Saxena. 1996. Registration of ILL 5588 lentil germplasmresistant to vascular wilt and ascochyta blight. Crop Sci. 36, 1080.
[10] Eujayl, I., Baum M., W. Erskine, E. Pehu and F.J. Muehlbauer. 1997. The use of RAPD markers for lentil geneticmapping and the evaluation of distorted F2 segregation.Euphytica 96: 405-412.
[11] FAOSTAT. 2005. Available at: (Retrieved on 23rd December 2012).
[12] Fikiru, E., K. Tesfaye and E. Bekele. 2010. A comperative study of morphological and molecular diversity in Ethiopean lentil (Lens culinaris Medikus) landraces. African journal of Plant Science 4: 242-254.
[13] Hamwieh, A., S.M. Udupa, A. Sarkar, C. Jung and M. Baum. 2009. Develpoment of new microsatellite markers and their application in the analysis of genetic diversity in lentils. Breeding Science 59: 77-86.
[14] Hamwieh, A., S.M. Udupa, W. Choumane, A. Sarker,F. Dreyer, C. Jung and M. Baum. 2005. A genetic linkagemap of Lens sp. based on microsatellite and AFLPmarkers and the localization of fusarium vascular wiltresistance. Theor. Appl. Genet. 110: 669-677.
[15] Hayward, M.D. and E.L. Breese. 1993. Population structure and variability. In:Plant Breeding: Principles and Prospects, (Eds.): M.D. Hayward, N.O. Bosemark and I. Romayosa, Chapman and Hall, London. pp. 17-29.
[16] Jha, S.S. and D. Ohri. 1996. Phylogenetic relationships of Cajanus cajan(L.)Millsp. (Pigeonpea) and its wild relatives based on seed proteinprofiles. Genet. Resour. Crop Evol. 43: 275-281.
[17] Linacero, R.J., Rueda and A.M. vazquez. 1998. Quantifying of DNA. Karp, A., P.G. Isaac, and D.S. Ingram (eds.) molecular tools for screening biodiversity: plants and animals. Chapman and hall. London, Weinheim, new york, Tokyo, Melbourne, madras. pp. 18-21.
[18] Margale, E., Y. Herve, J. Hu and C.V. Quiros. 1995. Determination of geneticvariability by RAPD markers in cauliflower, cabbage and kale localcultivar from France. Genet. Resour. Crop Evol. 42: 281-289.
[19] MoAD.2011. Stastistical Information on Nepalese Agriculture 2010-11. Government of Nepal, Ministry of agriculture and Development, Agribusiness promotion and Stastistics Division, Singh Darbar, kathmandu, Nepal. Availabel at: (Retrieved on 10th January 2013).
[20] Nazir, S., E. Bashir, R. Bantel and Habib-ul-Rehman Mian. 1994. Crop Production. NationalBook foundation, Islamabad. pp. 294-300.
[21] Nienhuis J., Tivang J. and Skroch P. 1995. Genetic relationship amongcultivars and landraces of lima bean (Phaseolus lunatusL.) asmeasured by RAPD markers. J. Amer. Soc. Hort. Sci. 120: 300-306.
[22] Sambrook, J., E.F. Fritch and T. Manniatis. 1989. Molecular cloning: A laboratory manual, (2nd edition), Cold Spring harbor Laboratory Press, Cold spring Harbor, NY.
[23] Savage, G.P. 1988. The composition and nutritive value oflentils. Nature Abstract Review 58: 319–343.
[24] Sneath, P.H.A. and R.R. Sokal. 1973. Numerical taxonomy. Freeman, San Francisco.
[25] Sonnante, G. andD. Pignone. 2001. Assessment of genetic variation in a collection of lentilusing molecular tools. Euphytica 120: 301–307.
[26] Virk, P.S., B.V. Ford-Lloyd, M.T. Jackson and H. John. 1995. Use of RAPD forthe study of diversity within plant germplasm collections. Heredity 74:170-179.
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