Agricultural and Biological Sciences Journal
Articles Information
Agricultural and Biological Sciences Journal, Vol.5, No.1, Mar. 2019, Pub. Date: Apr. 29, 2019
Germination and Mitochondrial Respiration Rate in Vigna unguiculata (Bean) Seeds Exposed to Crude Oil
Pages: 29-34 Views: 1195 Downloads: 317
Authors
[01] Stella Oghomwen Olubodun, Department of Medical Biochemistry, School of Basic Medical Sciences, University of Benin, Benin City, Nigeria.
[02] George Edaghogho Eriyamremu, Department of Biochemistry, Faculty of Life Sciences, University of Benin, Benin City, Nigeria.
Abstract
Vigna unguiculata (bean) seeds were treated with 0%, 2%, 5% 10% crude oil fractions [whole crude (WC), water soluble fraction (WSF) and water insoluble fraction (WIF)] for 21 days. Crude oil stress in some plant species leads to the formation of reactive oxygen species (ROS). However, evidence are lacking and very limited studies explore the measurement of mitochondrial respiration in developing plants during environmental stress. This study investigated the growth and mitochondrial respiration rate in bean seeds exposed to crude oil. Experiments carried out in equal amounts of developing roots after 7, 14, and 21 days post germination (DPG) were performed to determine plant height, radicle/root length and mitochondrial respiration rate. After 21 DPG, seedling height of bean in 10% WC, WSF, and WIF decreased significantly. Radicle/root lengths of bean seedlings in 10% WC, WSF and WIF at 7 DPG, also decreased. Decreased respiration rate was observed in the roots of bean in different crude oil fractions when compared with control but this was not found to be significant (P<0.05) with increase in crude oil contamination or increase in DPG. In conclusion, the germinating seedlings revealed reduced growth and development with display of morphological and biochemical variations in the radicle/roots in crude oil stress. We suggest that reduction of root length and mitochondrial respiration rate of the bean seedlings may be responsible for the observed decrease in growth and development in crude oil stress. There is a need for public enlightenment on the deleterious effects of crude oil spills in the environment and law for proper clean-up.
Keywords
Crude Oil Stress, Germination, Mitochondrial Respiration Rate, Vigna unguiculata (Bean)
References
[01] Miao He, Chong Zhu, Kun Dong, Ting Zhang Zhiwei Cheng, Jiarui Li and Yueming Yan (2015) Comparative proteome analysis of embryo and endosperm reveals central differential expression proteins involved in wheat seed germination. BMC Plant Biology, 15: 97.
[02] Jang DH, Greenwood JC, Spyres MB and Eckmann DM (2016) Measurement of Mitochondrial Respiration and Motility in Acute Care: Sepsis, Trauma, and Poisoning. Journal of Intensive Care Medicine, 32 (1): 86-94 https://doi.org/10.1177/0885066616658449.
[03] Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLoS ONE 12 (11): e0187523. https://doi.org/10.1371/journal.pone.0187523.
[04] Tiwari BS, Belenghi B and Levine A (2002) Oxidative Stress Increased Respiration and Generation of Reactive Oxygen Species, Resulting in ATP Depletion, Opening of Mitochondrial Permeability Transition, and Programmed Cell Death. Plant Physiology, 128 (4): 1271–1281. doi: 10.1104/pp.010999.
[05] Meyer JN, Hartman JH, and Mello DF (2018) Mitochondrial Toxicity. Toxicological Sciences, 162 (1): 15–23.
[06] Smolina N, Bruton J, Kostareva A and Sejersen T (2017) Assaying Mitochondrial Respiration as an Indicator of Cellular Metabolism and Fitness. Methods in Molecular Biology, 601: 79-87. doi: 10.1007/978-1-4939-6960-9_7.
[07] Logan DC, Millar AH, Sweetlove LJ, Hill SA and Leaver CJ (2001) Mitochondrial Biogenesis during Germination in Maize Embryos. Plant Physiology, 25 (2): 662–672.
[08] Attucci S., Carde J. P., Raymond P., Saint-Ges V., Spiteri A. and Pradet A. (1991). Oxidative phosphorylation by mitochondria extracted from dry sunflower seeds. Plant Physiology, 95: 390–398.
[09] Raymond P, Al-Ani A and Pradet A (1985) ATP production by respiration and fermentation, and energy charge during aerobis and anaerobis in twelve fatty and starchy seeds. Plant Physiology, 79: 879–884.
[10] Olubodun SO and Eriyamremu GE (2013) Effect of different crude oil fractions on growth and oxidative stress parameters of maize radicle. International Journal of Plant and Soil Science, 2 (1): 144-154.
[11] Olubodun SO and Eriyamremu GE (2018) Heavy metal concentrations in soil and morphological changes in Zea mays (maize) exposed to crude oil. Journal of Environmental Toxicology and Pollution Mitigation, 2: 38-46.
[12] Anderson JA, Huprikar SS, Kochain LV, Lucas WJ and Gaber RF (1992) Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae. Protocol of National Academy of Science, USA, 89: 3736–3740.
[13] Vavrek MC and Campbell WJ (2002) Identification of plant traits that enhance biodegradation of oil; utulsa.edu/conf. pp. 20.
[14] Douce RD, Bourgurgnon J, Brouguisse R and Neuburger M (1987) Isolation of plant mitochondria. General principles and criteria of integrity. Meth. Enzymol. 148: 403 – 409.
[15] North JA, Rein D and Tappel AL (1996) Multicomponent analysis of heme protein spectra in biological materials. Anal. Biochem. 233 (1): 115-123.
[16] Merckl N, Schutze-Kraft R and Arias M (2005) Influence of fertilizer level on Phytoremediation of crude oil-contaminated soils with the tropical grass Brachiaria brizantha (Hochst. ex A. Rich.) Stapf. In: Phytoremediation of petroleum-contaminated soil. Merkl, N. (Ed), Margraf Publisher, Weikershim, pp 71-83.
[17] Minai-Tehrani D and Mohammadi MK (2014) Crude Oil-polluted Soil Induces Ultrastructural and Enzyme Activity Changes in the Shoot of Lentil. J Stress Physiol. Biochem., 10 (1): 112-121.
[18] Omosun G, Markson AA and Mbanasor O (2008) Growth and anatomy of Amaranthus hybridus as affected by different crude oil concentrations. Am-Euras. J. Sci. Res., 3: 70-74.
[19] Henner P, Schiavan M, Druelle V and Lichtfouse E (1999) Phytotoxicity of ancient gas work soils. Effect of polycyclic aromatic hydrocarbons (PAHs) on plant germination. Org. Geochem. 30: 963–969.
[20] Nicolotti G and Eglis S (1998) Soil contamination by crude oil: impact on the Mycorrhizosphere and on the revegetation potential of forest trees. Environ Pol. 99: 37-43.
[21] Vwioko DE and Fashemi DS (2005) Growth response of Ricinus communis L. in spent lubricating oil polluted soil. J. Appl. Sci. Environ. Mgt., 9: 73-79.
[22] Atkin OK, Bruhn D, Hurry VM and Tjoelker MG (2005) The hot and cold: unraveling the variable response of plant respiration to temperature. Fnal. Plant Bio. 32, 87-105.
[23] Debska K, Krasuska U, Budnicka K, Bogatek R, and Gniazdowska A (2013) Dormancy removal of apple seeds by cold stratification is associated with fluctuation in H2O2, NO production and protein carbonylation level. J. Plant Physiol. 170, 480–488.
[24] Farooq M, Wahid A, Kobayashi N, Fujita D and Basra SMA (2009) Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development, 29 185–212.
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