[01] Husain El-Fadaly, Department of Agricultural Microbiology, Faculty of Agriculture, Damietta University, Damietta, Egypt. [02] Sherif El-Kadi, Department of Agricultural Microbiology, Faculty of Agriculture, Damietta University, Damietta, Egypt. [03] Salah El-Kholy, Department of Agricultural Microbiology, Faculty of Agriculture, Damietta University, Damietta, Egypt.

Mediterranean sea water and fish samples of Ras El-Bar city in Damietta governorate, Egypt were examined. Morphological examination of fish obtained from sea water was examined. The highest value of BOD₅²⁰ was found during summer being 2.4 mgO₂/L while the lowest value was found during spring being 0.8 mgO₂/L. Lead, Arsines and Stannum did not presented in all seasons of all samples (water and fish). Cadmium and cupper did not presented in all fish samples, but presented in all water samples. The lowest value of cadmium in fish was in the spring being 0.014 ppm while the highest value was in winter being 0.028 ppm. The highest value of total bacterial count in sea water was found during summer being 20cfu/ml×10³, while the lowest value was in spring and winter being 0.01cfu/ml×10³. The highest value of total fungal count was in summer being 4cfu/ml×10³ while lowest value was in the winter being 0.0cfu/ml×10³. There were no bacterial growth on SS agar medium. The highest value of Staphylococci count was in summer being 20cfu/ml×10³. The highest value of Aeromonas count in summer being 50cfu/ml×10³. The highest value of coliform count was in autumn being 30cfu/ml×10³. The highest value of total bacterial count of fish muscles, fish intestine and fish surface were 1600, 3000 and 1000cfu/g×10³ in summer, spring and summer, respectively. The highest values of fungal count in muscles, intestine and fish surface were during summer, spring, being 0.4, 900 and 430cfu/g×10³, respectively. The highest values of Staphylococcal counts of fish muscles, intestine and fish surface were in summer, being 20, 80 and 200cfu/g×10³, respectively. The highest count of Aeromonas sp. was in fish muscles, intestine and surface during summer, being 40, 1700 and 10cfu/g×10³, respectively. Coliform was found in the highest values in summer being 36, 9.2 and 2400cfu/g×10³ in muscles, intestine and surface, respectively. A strong correlation coefficient value (r = 0.79) between the log of total bacterial count (LTBC) and BOD in the sea water was indicated, which means there are a highly pollution by industrial pollutants. Among 10 bacterial isolates, only one was coccoid shape, 4 isolates were short rods and 5 isolates were long rods. Four isolates were Gram negative and 6 isolates were Gram positive. Five isolates were spore formers and 5 isolates were non spore formers. All isolates gave negative results with acid fast stain. Micrococcus sp., Bacillus sp., Esherichia sp. Pseudomona sp., Aspergillus niger, and A. flavus were isolated and identified from water and fish.

Sea Water, Microbiological Examination, BOD, Correlation Coefficient, Isolation and Identification

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