[01] Hussein A. H. Said-Al Ahl, Department of Medicinal and Aromatic Plants, National Research Centre, 33 El Bohouth St. (former El Tahrir St.), Dokki, Giza, Egypt. [02] Atef M. Sarhan, Department of Ornamental Horticulture, Faculty of Agriculture, Cairo University, Cairo, Egypt. [03] Abou Dahab M. Abou Dahab, Department of Ornamental Horticulture, Faculty of Agriculture, Cairo University, Cairo, Egypt. [04] El-Shahat N. Abou Zeid, Department of Medicinal and Aromatic Plants, National Research Centre, 33 El Bohouth St. (former El Tahrir St.), Dokki, Giza, Egypt. [05] Mohamed S. Ali, Department of Medicinal and Aromatic Plants, National Research Centre, 33 El Bohouth St. (former El Tahrir St.), Dokki, Giza, Egypt. [06] Nabila Y. Naguib, Department of Medicinal and Aromatic Plants, National Research Centre, 33 El Bohouth St. (former El Tahrir St.), Dokki, Giza, Egypt.

This study was to investigate the response of Anethumgraveolens L. plants to the nitrogen fertilizer (0, 20, 40, 60 and 80kg N/fed.) with or without bio-fertilizers (at 2kg/fed.) on the flavonoids content and essential oil as well as its chemical constituents during the vegetative stage (90 days after sowing). The recorded results showed that in the two seasons, treatment of 60kgN/fed. with or without bio-fertilizers gave the best values of volatile oil percentage and oil yield in the herb, Also, the most effective fertilization treatment on flavonoids content was 40 or 60 Kg N/fed., with bio-fertilizers. In the essential oil constituents of dill herb, all treatments showed α-phellandrene (42.44 to 66.39%)as the major compound followed by p-cymene(11.97to 18.39%) and limonene(1.42 to 14.25%)then -phellandrene (7.67 to 11.18%).

Anethum graveolens L., Flavonoids, Essential Oil, Nitrogen, Bio Fertilizer

[01] Al-Humaid, A.I. (2004).Effect of bio and chemical fertilizers on the growth and essential oil yield of fennel (FoeniculumvulgareMill.). Alex. J. Agric. Res., 49 (3): 83-91. [02] Al-Qadasi, A.S.S. (2004).Effect of biofertilization on Ocimumbasilicum L. plants. M.Sc. Thesis, Fac. of Agric., Cairo Univ. [03] Bailer, J., Aichinger, T., Hackl, G., Hueber, K. D. and M. Dachler (2001). Essential oil content and composition incommercially available dill cultivars in comparison to caraway. Industrial Crops and Products, 14:229-39. [04] Barbara, K. (2002).The effect of soil material and nitrogen fertilization on growth and development of goldenrod (Solidagovirga-aureaL.). Folia Hort., 14 (1): 187-193. [05] Bhati, D.S. and M.S. Shaktawat (1994).Effect of sowing date, row spacing and nitrogen on quality parameters of coriander (coriandrumsativumL.). Progressive Hort., 26 (1/2): 14-18. [06] Borella, J.C.; A. Fontoura; A. Menezes Junior and S.C. Franca (2001). Effect of mineral fertilization (N-P-K) and seasonality in yield and total flavonoids in male individuals of Baccharistimeraless. (Asteraceae)-Carqueja. [Portuguese]. Revista Brasileira de plantas Medicinais, 4: 1, 99-102. [07] Frąs, B. (2009). Comparison of eight dill cultivars grown in containers in different lightconditions.HerbaPolonica, 55 (3):76-83. [08] Guenther, G. (1961). “The essential Oils” V. III. Robert E.D. Nastrand Comp. Inc. Toronto, New York, London. [09] Halva, S., Huopalahti R. and S. Makinen (1987). Studies on fertilization of dill (Anethumgraveolens L.) and basil (Ocimumbasilicum L.). II. Oil yield of dill and basil as affected by fertilization. J. Agric. Sci., in Finland 59 (1): 19-24. [10] Kandeel, Y. M., Menesy, F. A., Khalafalla, M. M. and W.M. Gad (2004, a). Effect of some comercialbiofertilizers on growth, seed, volatile oil yield and chemical composition of Anethumgraveolens L. J. Agric. Res. Tanta Univ., 30 (3): 705-720. [11] Kandeel, Y. M., Menesy, F. A., Khalafalla, M. M. and W.M. Gad (2004, b).Effect of some comercialbiofertilizers on growth, seed, volatile oil yield and chemical composition of Foeniculumvulgare Mill. J. Agric. Res., Tanta Univ., 30 (3): 721-737. [12] Kolar, L., Ledvina, R., Kuzel, S. and J. Pasek (1998). The effect of nitrogen surplus in fertilizer rates applied to Echinacea Purpurea (L.) Moench. on the production of its active substances. Rostlinna Vyroba, 44(11): 489-495. [13] Kovac-Besovic, E. E. and K. Duric (2003).Thin layer chromatography-application in qualitative analysis on presence of coumarins and flavonoids in plant material. Bosnian J. Basic Med. Sci., 3 (3): 19-26. [14] Letchamo, W. (1992).A comparative study of chamomile yield, essential oil and flavonoids content under two sowing seasons and nitrogen levels. Acta Hort., 306: 375-384. [15] Mabry, T. J., Markham, K. R. and M.B. Thomas (1970).The systematic identification of flavonoids. Springer-Verlag. [16] Rupam, K., Giri, B. and K.G. Mukerji (2001). Effect of vesicular arbuscular mycorrhiza on growth and essential oil yield of Anethumgraveolens L. Indian J. Plant Physiol., 6 (1): 77-80. [17] Said-Al Ahl, H. A. H., Hussein, M. S. and A. A. Abd El-Kader (2010).Effect of nitrogen fertilizer and/or some foliar applicationon growth, herb yield, essential oil and chemical composition of dragonhead. Journal of Medicinal Food Plants, 2(1): 12-28. [18] Score, C., Lorenzi, R. and P. Ranall (1997).The effect of (S)-(+)-carvone treatments on seed potato tuber dormancy and sprouting. Potato Research, 40:155-161. [19] Simon, J.E., Chadwick, A.F. and L.E. Craker (1984). Herbs. Archon Books, Hamden, CT 1984:32. [20] Singh, A., Randhawa, G. S., Mahey R. K. and A. Singh (1993). Growth and yield of dill (Anethumgraveolens L.) as affected by nitrogen and harvesting stages. Crop Res., (Hisar) 6 (2): 511-221. [21] Steel, R. G. D. and S. H. Torrie (1980). Principales and Procedure of Statistics. Second Edition, Mc Grow Hill Inc. [22] Youssef, A. A., Edris, A. E. and A.M. Gomaa (2004). Acomparative study between some plant growth regulators and certain growth hormones producing microorganisms on growth and essential oil composition of Salvia officinalis L. plants. Ann. Agric. Sci., Ain Shams Univ., Cairo, 49 (1): 299-311. [23] Varshney, S.C. (1991).Agro-technology with respect to quality control of essential oils. Indian Perfumer, 35 (4): 241-247. [24] Venskutonis, R., Kvietkauskaite, D., Bylaite, E. and A. Siuliauskas (1999). Characterization of caraway (Carumcarvi L.) cultivated in Lithuania. Sodininkysteir Dar zininkyste, 18 (3): 85-92. [25] Volkhonskaya, T. A. (1984). [Influence of growing condition of Bupleurum L. species on flavonoid content and flavonoid splitting enzyme activity.]. Rastitel’ nye Resursy, 20 (1): 106-113. (C.F. Hort. Abst., 1984 Vol. 54, No. 8: 5738). [26] Weiss, E.A. (2002). Spice Crops. CABI Publishing, 10E 40th street, Suite 3203, New York, NY 10016 U.S.A.