[01] Razia Khatun, Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh. [02] Md. Abdul Jabbar Howlader, Department of Zoology, Jahangirnagar University, Savar, Dhaka, Bangladesh. [03] Md. Nazrul Islam, Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh. [04] Md. Khorshed Alam, Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh. [05] Mohammad Showkat Mahmud, Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh. [06] Md. Hafizur Rahman, Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh.

The present study was designed to exploration the causes of infectious illness and detection of antibiotic residues in warehouse poultry of Sirajgonj district, Bangladesh. A total of 96 samples including 24 live broiler birds, 24 samples of poultry litters, water and feed (24 x3=72) were collected from different warehouse of study areas. Among the 96 samples, 24 live broilers were subjected to post mortem examination followed by histopathological examination; in which 5(21%) were suspected to positive for Salmonella spp. and 3(13%) were Mycoplasma spp. infection. After that serum samples from these birds were tested and positive for agglutination test using specific antiserum of Salmonella pullorum (SP) and Mycoplasma gallisepticum (MG). In addition 24 samples of litters, water and feed were also tested by microbiological examination (Culture and biochemical test) for exploration the causes of infectious illness in warehouse birds. The microbiological study showed that 91% (22/24) litter samples were infected by E. coli and 83% (20/24) by Salmonella spp.; 75% (18/24) water samples had mixed infection with E. coli and Salmonella spp.; and 25% (6/24) feed samples by Salmonella spp. and 33% (8/24) by E. coli. The ELISA test from meat samples of 24 live broiler birds showed that 16.70% (4/24) breast muscle has residues for Sulfadiazine and Oxytetracycline; 29.17% (7/24) thigh muscle for Oxytetracycline and 16.70% (4/24) for Sulfadiazine; 20.83% (5/24) liver for Oxytetracycline and Sulfadiazine; 29.17% (7/24) for Ciprofloxacin and Enrofloxacin, 8.33% (2/24) for Chloramphenicol and Furazolidone. No Neomycin or Gentamycin residues were detected in all tested samples. The above results showed that bacterial loads in the items used at birds selling centers were high and the content of different drug residues in poultry meat showed great concern for public health ground and need to be more attention.

Poultry, Antimicrobial Residues, Microorganisms, Warehouse

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