Clinical Medicine Journal
Articles Information
Clinical Medicine Journal, Vol.3, No.4, Aug. 2017, Pub. Date: Nov. 16, 2017
Detection of Epstein-Barr Virus in Oral Squamous Cell Carcinoma at Khartoum
Pages: 30-34 Views: 134 Downloads: 60
Authors
[01] Alfrazdag Mohamedahmed Abdalla Abdalrazig, Department of Oral and Maxillofacial Surgery, Khartoum Teaching Dental Hospital, Khartoum, Sudan.
[02] Salah Eldin G Elzaki, Institute of Tropical Medicine, Khartoum, Sudan.
[03] Elneel Ahmed Mohamed, Department of Oral and Maxillofacial Surgery, Khartoum Teaching Dental Hospital, Khartoum, Sudan.
Abstract
Oral squamous cell carcinoma consider the most common type of oral cancer and carry poor prognosis especially in late stages, many factors like tobacco, alcohol, poor diet, have been investigated for their relation to this cancer however many patient develop oral cancer without exposure to these factors, this rise question about other factors may contribute to squamous cell carcinoma this factors include oncogenic viruses like human papilloma virus, herpes simplex virus and EPSTEIN-BARR VIRUS. Experimental cross-sectional study in Khartoum teaching dental hospital sudan and institute of tropical medicine, to detect presence of EPSTEIN-BARR VIRUS in oral squamous cell carcinoma samples using PCR, a total of 107 samples taken from oral squamous cell carcinoma patients 66 (61.7%) are male while 41 (38.3) are female. Mean age of the patient is 57. Forty four patients are snuffer while 63 are not. 17 patients are smoking while 90 patients are not. Only 7 patients consume alcohol. most of the patients are laborers and house wife. The patients came from all site of the Sudan with slight majority from central of the Sudan. DNA extracted and PCR used for detection. Total of 107 O.S.C.C samples tested by PCR for detection of EPSTEIN-BAAR VIRUS 35 (32.7%) samples are positive while 72 (67.3%) samples are negative. About 45 lesions in the lower jaw, 9 lesions in the lip, 20 lesions in buccal mucosa, 16 lesions in the upper jaw, 15 lesions in the tongue and only two lesions in the flour of the mouth. The 44 patients with snuffing habit show 20 patient positive for EPSTEIN-BAAR VIRUS while 63 non snuffer patients show 15 patients positive for EBV and this result show statistically significant different with P value (0.019). This study result revealed that EPSTEIN-BAAR VIRUS could be one of the causative factors that lead to squamous cell carcinoma especially in the patient with snuffing habbite.
Keywords
Oral Squamous Cell Carcinoma, EBV, PCR, Khartoum, Sudan
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