International Journal of Animal Biology
Articles Information
International Journal of Animal Biology, Vol.1, No.4, Aug. 2015, Pub. Date: Jul. 3, 2015
In Vitro Selection of Bacteria and Isolation of Probionts from Farmed Sparus aurata with Potential for Use as Probiotics
Pages: 93-98 Views: 3322 Downloads: 1327
Authors
[01] Mancuso M., Institute for Coastal Marine Environment (IAMC), National Research Council (CNR), Section of Messina, Italy.
[02] Rappazzo A. C., Institute for Coastal Marine Environment (IAMC), National Research Council (CNR), Section of Messina, Italy.
[03] Genovese M., Institute for Coastal Marine Environment (IAMC), National Research Council (CNR), Section of Messina, Italy.
[04] El Hady M., Department of Fish Diseases and Management, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
[05] Ghonimy A., Institute of Oceanography and Fisheries (NIOF), Cairo, Egypt.
[06] Ismail M., Center of Excellence for Advanced Sciences (CEAS), Environmental Research Division National Research Center (NRC), Cairo, Egypt.
[07] Reda R., Department of Fish Diseases and Management, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
[08] Cappello S., Institute for Coastal Marine Environment (IAMC), National Research Council (CNR), Section of Messina, Italy.
[09] Genovese L., Institute for Coastal Marine Environment (IAMC), National Research Council (CNR), Section of Messina, Italy.
[10] Maricchiolo G., Institute for Coastal Marine Environment (IAMC), National Research Council (CNR), Section of Messina, Italy.
Abstract
In the last years aquaculture has contributed significantly to reduce the hunger worldwide. One of the major treats in the development of a massive production is linked to the outbreak of diseases and the abuse of antibiotics, that is to avoid because of the acquisition of antibiotic resistance. For these reasons recently probiotics are used as alternative measures to control the fish diseases. Fish possess specific intestinal microbiota consisting of aerobic, facultative anaerobic and obligate anaerobic bacteria so we make this study in order to find some probiotic candidates that have an antagonistic action against fish pathogens. Adults of Sparus aurata farmed in intensive plant were sacrificed and 40 bacterial strains were isolated from GI tract. All the strains were tested against three fish pathogens: Vibrio anguillarum, Photobacterium damselae subsp. piscicida and Pseudomonas anguilliseptica. Results showed that only 3 candidates respectively called (SA7, SA10, and SA20) showed an inhibitory activity against the selected fish pathogens bacteria. The candidates probionts were identified by the 16S rRNA gene sequenced-based. The three candidates inhibited the Gram negative fish pathogens after 24 -48 h of incubation at 24°C and for these reasons could be used as probiotics to added into the food to enhance the immune defence of fish.
Keywords
Antimicrobial Effects, Fish Pathogens, Microbial Gut, Probiotics, Seabream
References
[01] Balcázar J.L., Vendrell D., De Blas I., Ruiz-Zarzuela I., Muzquiz J.L., Girones O., 2008. Characterization of probiotic properties of lactic acid bacteria isolated from intestinal microbiota of fish. Aquaculture, 278, 188-191.
[02] Biebl H., Pukall R., Lundsdorf H., Schulz S. et al., 2007. Description of Labrenzia alexandrii gen. nov., sp. nov., a novel alphaproteobacterium containing bacteriochlorophyll a, and a proposal for reclassification of Stappia aggregata as Labrenzia aggregata comb. nov., of Stappia marina as Labrenzia marina comb. nov. and of Stappia alba as Labrenzia alba comb. nov., and emended descriptions of the genera Pannonibacter, Stappia and Roseibium, and of the species Roseibium denhamense and Roseibium hamelinense. Int. J. Syst. Evol. Microbiol., 57, 1095-1107.
[03] Cahill MM., 1990. Bacterial flora of fishes: a review. Microbial Ecol. 19, 21-41.
[04] Chevassus B. and Dorson. M., 1990. Genetics of resistance to disease in fish. Aquaculture, 85,83-107.
[05] Das S., Lyla P.S., Ajmal Khan S. 2006. Marine microbial diversity and ecology: importance and future perspectives. Curr Sci., 25, 1325- 1335.
[06] Decamp O., Moriarty D. 2007. Aquaculture species profit from probiotics. Feed Mix, 1, 20-23.
[07] Denev S.A.,1996. Probiotics – Past, Present and Future. Bulgarian Journal of Agricultural Sciences, 2, 445-474.
[08] Denev S., Staykov Y., Moutafchieva R., Beev G., 2009. Microbial ecology of the gastrointestinal tract of fish and the potential application of probiotics and prebiotics in finfish aquaculture Int. Aquat. Res., 1, 1-29.
[09] Dopazo C., Lemos M., Lodeiros C., Bolinches J., Barja J., Toranzo A., 1988. Inhibitory activity of antibiotic-producing marine bacteria against fish pathogens. J. Appl. Bacteriol. 65, 97–101.
[10] Floris R., Manca S., Fois N., 2013. Microbial ecology of intestinal tract of gilthead sea bream (Sparus aurata Linnaeus, 1758) from two coastal lagoons of Sardinia (Italy) Transitional Waters Bulletin, 2, 4-12.
[11] Genovese M., Crisafi F., Denaro R., Cappello S., et al., 2014. Effective bioremediation strategy for rapid in situ cleanup of anoxic marine sediments in mesocosm oil spill simulation Frontiers in Microbiology - doi: 10.3389/fmicb.2014.00162
[12] Giri S.S., Sen S.S., Sukumaran V., 2012. Effects of dietary supplementation of potential probiotic Pseudomonas aeruginosa VSG-2 on the innate immunity and disease resistance of tropical freshwater fish, Labeo rohita. Fish and Shellfish Immunology, 32,1135–1140.
[13] Giri S.S., Sukumaran V., Oviya M., 2013. Potential probiotic Lactobacillus plantarum VSG3 improves the growth, immunity, and disease resistance of tropical freshwater fish, Labeo rohita. Fish and Shellfish Immunology, 34, 660–666.
[14] Gomez G.D., Balcazar J.L., 2008. A review on the interactions between gut microbiota and innate immunity of fish. FEMS Immunol Medical Microbiol, 52, 145-154.
[15] Guarner F., Malagelada J.R., 2003. Gut flora in health and disease. The Lancet 36,512-519.
[16] Gutowska M.A., Drazen J.C., Robison B.H. 2004. Digestive chitinolytic activity in marine fishes of Monterey Bay, California. Comp Biochem and Physiol., 139, 351-358.
[17] Kesarcodi-Watson A., Kaspar H., Lategan M.J., Gibson L., 2008. Probiotics in aquaculture: The need, principles and mechanisms of action and screening processes. Aquaculture, 274, 1-14.
[18] Lane D.J.,1991. 16/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics ed. Stackebrandt, E. and Goodfellow, M. pp. 115-175. New York: Wiley.
[19] Maidak B.L., Olsen G. J., Larsen N., Overbeek R., Mc Caughey M.J., Woese C.R., 1997. Nucleic acid Research, 25, 109-111.
[20] Mancuso M., Basile V., Innella G., Marino F., Cavalieri S., Zaccone R., 2005. Mugil cephalus: un campanello d’allarme della comparsa di focolai di Pseudotubercolosi in Spigole allevate in gabbie off-shore” Biologia Marina Mediterranea, 12, 195-197.
[21] Mancuso M., 2012. Photobacteriosis exchange between wild and farmed fish in the Mediterranean area. Journal of aquaculture research and development, 3, 3-6.
[22] Mancuso M., Caruso G., Adone R., Genovese L., Crisafi E., Zaccone R., 2013. Detection of Photobacterium damselae subsp. piscicida in seawaters by fluorescent antibody. Journal of Applied Aquaculture, 25, 337 – 345.
[23] Mancuso M., 2013a. Probiotics in aquaculture. Journal of Fisheries and livestock production (JFLP) 1:e107doi: 10.4172/2332-2608.1000e107
[24] Mancuso M., 2013b. Aquaculture advancement. Special Issue “Fisheries and Aquaculture advancement” to Journal of Aquaculture Research and Development doi:10.4172/2155-9546.1000e1080
[25] Mancuso M., 2013c. Fish welfare in aquaculture. Journal of Aquaculture Research and Development, 3, 4-6.
[26] Mancuso M., 2014. Emerging bacterial diseases in Mediterranean Mariculture. Journal of Aquaculture and Research, 1, 1-2.
[27] Maricchiolo G., Caccamo L., Mancuso M., Cusimano G.M., Gai F., Ghonimy A., Genovese M., Genovese L., 2015. Saccharomyces cerevisiae var. boulardii preserves the integrity of intestinal mucosa in gilthead seabream, Sparus aurata subjected to a bacterial challenge with Vibrio anguillarum. Aquaculture Research (in press).
[28] Maricchiolo G., Guerrera MC., Castex M., Gai F., Parisi V., Perdichizzi A., Genovese L., Gangemi S., 2014. Effects of Saccharomyces cerevisiae var. boulardii on interleukin-1β expression and gut morphometry in European sea bass: preliminary results. Proceeding of the International Conference Aquaculture Europe ’14: Adding Value. Donostia-San Sebastian, Spain 14-17 October, pp 769-770.
[29] Martínez Cruz P., Ibáñez A.L., Monroy Hermosillo O.A., Ramírez Saad H.C., 2012. Use of Probiotics in Aquaculture SRN Microbiology, Article ID 916845, 13 pages.
[30] Martinez-Porchas M. and Martinez-Cordova M.R., 2012. World Aquaculture: Environmental Impacts and Troubleshooting Alternatives. Scientific World Journal.
[31] Mukherjee A. and Ghosh K., 2014. Antagonism against fish pathogens by cellular components and verification of probiotic properties in autochthonous bacteria isolated from the gut of an Indian major carp, Catla catla (Hamilton). Aquaculture Research, 1-13.
[32] Nomoto K., 2005. Prevention of infections by probiotics - Journal of Bioscience and Bioengineering, 100, 583–592.
[33] Panigrahi A. and Azad I.S., 2007. Microbial intervention for better fish health in aquaculture: the Indian scenario. Fish Physiology Biochem., 33, 429-440.
[34] Ram C.S. and Parvati S., 2012. Probiotics: the new ecofriendly alternative measures of disease control for sustenaible aquaculture. J. of fish and Aquatic sciences, 7, 72-103.
[35] Ringø E. and Gatesoupe F. J., 1998. Lactic acid bacteria in fish: a review. Aquaculture, 160, 177-203.
[36] Salminen S.J., Gueimonde M., Isolauri E., 2005. Probiotic that modify disease risk. J. Nutr., 135, 1294-1298.
[37] Soccol C.R., Porto de Souza Vandenberghe L., Rigon Spier M., Bianchi Pedroni Medeiros A., Tiemi Yamaguishi C., De Dea Lindner J., Pandey A., Thomaz-Soccol V., 2010. The Potential of Probiotics, Food Technol. Biotechnol., 48, 413–434.
[38] Subasinghe R., Soto D., Jia J., 2009. Global aquaculture and its role in sustainable development. Reviews in Aquacul., 1: 2-9.
[39] Sugita H., Hirose Y., Matsuo N., Deguchi Y., 1998. Production of the antibacterial substance by Bacillus sp. strain NM 12, an intestinal bacterium of Japanese coastal fish. Aquaculture 165, 269–280.
[40] Vendrell D., Balcázar J.L., Ruiz-Zarzuela I., De Blas I., Muzquiz J.L. 2006. Lactococcus garvieae in fish: a review. Comp. Immunol. Microbiol. Infec. Dis., 29, 177-198.
[41] Verschuere L., Rombaut G., Sorgeloos P., Verstraete W. 2000. Probiotic bacteria as biological control agents in aquaculture. Microbiology and Molecular Biol. Rev. 64, 655-671.
[42] Vijayan K.K., Bright Singh I.S., Jayaprakash N.S., Alavandi S.V., Somnath Pai S., Preetha R. , Rajan J.J.S., Santiago T.C., 2006. Brackishwater isolate of Pseudomonas PS-102, a potential antagonistic bacterium against pathogenic vibrios in penaeid and non-penaeid rearing systems. Aquaculture, 251, 192-200.
[43] Vine N.G., Leukes W.D., Kaiser H., 2004a. In vitro growth characteristics of five candidate aquaculture probiotics and two fish pathogens grown in fish intestinal mucus. FEMS Microbiol Letters, 231, 145-152.
[44] Yakimov M.M., Cappello S., Crisafi E., Tursi A., Corselli C., Scarfì, S., Giuliano L., 2006. Phylogenetic survey of metabolically active microbial communities associated with the deep-sea coral Lophelia pertusa from the Apulian Plateau, Central Mediterranean Sea. Deep Sea Res Part I,53, 62-75.
[45] Yoshimizu M, Ezura Y. 1999. Biological Control of Fish Viral Diseases by Anti-Viral Substance Producing Bacteria. Microbes and Environ., 14, 269-275.
[46] Zaccone R., Caruso G., Zampino D., Mancuso M., Genovese L., Adone R., Ciuchini F., Manfrin A., 2004. Early detection of Vibrio anguillarum in waters: a challenge experience on Dicentrarchus labrax in microcosm, Acts of Aquaculture Europe Special Publication, 34, 849-850.
[47] Zhou A., Liu Y., Shi P., He S., Yao B., Ringø E., 2009. Molecular characterization of the autochthonous microbiota in the gastrointestinal tract of adult yellow grouper (Epinephelus awoara) cultured in cages. Aquaculture, 286, 184-189.
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