International Journal of Animal Biology
Articles Information
International Journal of Animal Biology, Vol.1, No.4, Aug. 2015, Pub. Date: Jul. 15, 2015
Determination of Humoral Immune Response in Chickens Against Formalin-Inactivated Alum-Precipitated Fowl Cholera Vaccine
Pages: 114-117 Views: 3077 Downloads: 1015
[01] Md. Zulfekar Ali, Department of Laboratory and Health Management, Nourish Poultry and Hatchery Ltd. Dhaka, Bangladesh.
[02] Shirin Sultana, Department of Livestock Services, Dhaka, Bangladesh.
We measured the humoral immune response in chickens against a formalin-inactivated alum-precipitated fowl cholera vaccine. The vaccine was administered in 12 weeks old chickens, 5×107CFU/ml/chicken intramuscularly. Booster dose was given with similar dose and route at 15, 30 and 45 days intervals in groups A, B and C, respectively, after primary vaccination. The group D served as unvaccinated control. Pre-vaccination sera were collected from all the groups of birds. Sera of the immunized and control birds were collected at 15, 30, 60, 90, 120, 150 and 180 days post vaccination (DPV). The degree of immunity produced in each group of birds following primary and secondary vaccination were measured by determining their serum antibody level using passive Haemagglutination (PHA) test. Sera sample possessing higher PHA titers after 15 days of primary vaccination manifested a declining tendency at 30 DPV and rapidly fell down at 60 DPV. Boostering of vaccination at this stage elucidated a rapid increase of PHA titers ranging from 128 to 256 at 30 DPV and declined gradually from 60 DPV and reached a titer of approximately 8 to 4 at 180 DPV. Challenge infection was conducted with five randomly selected vaccinates of all the groups of birds along with unvaccinated controls after 15 days of secondary vaccination. The fowl cholera vaccination conferred 100% protection while all the unvaccinated control birds succumbed to such infection.
Chickens, Humoral Immune, Fowl Cholera Vaccine
[01] Akand M. S. I., Choudhury K. A., Kabir S. M. L., Sarkar S. K. and Amin K. M. R. 2004. Development of Washed Cell Fowl Cholera Vaccine in Bangladesh. International Journal of Poultry Science, 3: 534-537.
[02] Baki M. A., Islam M. P., Das P. M., Karmaker P. K. and Mondal M. M. H. 1991. Pathology of duck cholera in natural and experimental infection. Bangladesh Journal of Microbiology, 8: 1-4.
[03] Bhasin, J. L and Biberstein, E. L. 1968. Fowl cholera in Turkeys-the efficacy of adjuvant bacterin. Avian Disease, 12:159-168.
[04] Choudhury K. A., Amin M. M., Rahman A. and Ali R. 1985. Investigation of natural outbreaks of fowl cholera. Bangladesh Veterinary Journal, 19: 49-56.
[05] Chowdhury, K. A, Amin, M. M, Rahman A and Ali, M. R. 1986. Serotyping of Pateurella multocida isolated from chickens and ducks. Bangladesh veterinary Journal, 20:55-63.
[06] Chowdhury, K. A, Amin, M. M, Rahman, A and Ali, M. R. 1985. Investigation of natural outbreak of fowl cholera. Bangladesh Veterinary Journal, 19:49-56.
[07] Coates, R. S, Jensen, J. M and Brown, E. D. 1977. The response of turkey to varying doses of live oral Pateurella multocida vaccine. Poultry Science, 56:273-276.
[08] Collins, F. M. 1977. Mechanisms of acquired resistance of Pateurella. Cornell Veterinary, 67:103-167.
[09] Dick J. W. and Avakian A. P. 1991. Response of broiler chickens to fowl cholera vaccination at 1 to 6 weeks of age. Avian Diseases, 35: 761-766.
[10] Dua, S. K and Maheswaran, S. K. 1978. Studies of Pateurella multocida between levels of immunity induced by various challenges. Avian Disease, 22:748-764.
[11] Glisson J. R., Hofacre C. L. and Christensen J. P. 2008. Fowl cholera. In: Diseases of Poultry, Saif Y. M., Barnes H. J., Glisson J. R., Fadly A. M., McDougald L. R. and Swayne D. E. (Editors). Blackwell Publishing, Ames, Iowa, USA, pp. 739-758.
[12] Hopkins B. A. and Olson L. D. 1997. Comparison of live avirulent PM-1 and CU fowl cholera vaccines in turkeys. Avian Diseases, 41: 317-25.
[13] Kasten R. W., Carpenter T. E., Snipes K. P. and Hirsh D. C. 1997. Detection of Pasteurella multocida specific DNA in turkey flocks by use of the polymerase chain reaction. Avian Diseases, 41: 676-682.
[14] Khan, M. A. H. N. A, Das, P. M, Choudhury, K. A and Islam, M. R. 1997. Pathology of experimentally induced fowl cholera vaccine in chickens. Bangladesh Veterinary Journal, 31:28-34.
[15] Khan, M. A. H. N. A, Das, P. M, Choudhury, K. A and Islam, M. R. 1994. Efficacy of alum precipitated fowl cholera vaccine in chickens. Bangladesh Veterinary Journal, 28:25-30.
[16] Kwon Y. K and Kang M. I. 2003. Outbreak of Fowl cholera in Baikal teals in Korea. Avian Diseases, 47: 1491-1495.
[17] Leonchuk, S. I and Tsimokh, P. P. 1977. Immunological changes in chickens vaccinated with emulsified Pasteurellosis vaccines. Veterinariya, Kiev Ukrainian, 43:36-39.
[18] Mondal, S. K, Choudhury, K. A, Amin, M. M, Rahman, M. M and Sarker, A. J. 1988. Immune response in chickens induced by alum precipitated fowl vaccine. I. Humoral immune response. Bangladesh Veterinary Journal, 22:63-69.
[19] Petersen K. D., Christensen J. P., Permin A. and Bisgaard M. 2001. Virulence of Pasteurella multocida subsp. multocida isolated from outbreaks of fowl cholera in wild birds for domestic poultry and game birds. Avian Pathology, 30: 27-31.
[20] Rahman M. B., Suman M. S. R., Kabir S. M. L. and Choudhury K. A. 2004a. Application of passive haemaglutination assay for the determination of efficacy of experimentally prepared formalin killed fowl cholera vaccine. The Bangladesh Veterinarian, 21: 1-8.
[21] Rahman M. K., Rahman M. B., Siddiky M. N. A., Kafi M. A., Islam M. A. and Choudhury K. A. 2004b. Efficacy of formalin killed fowl cholera vaccine in experimentally immunized Fayoumi chickens. Bangladesh Journal of Veterinary Medicine, 2: 23-25.
[22] Rana M., Hossain M. T., Islam M. A., Rahman M. M., Alam M. K. and Dutta U. K. 2010. Comparative immunogenicity study in ducks of different breeds available at coastal regions of Bangladesh against duck plague and duck cholera vaccines International Journal BioResearch, 2: 23-27.
[23] Samad M. A. 2000. Veterinary Practitioners Guide. 1st Pub. LEP Pub. No. 7, BAU Campus, Mymensingh.
[24] Sander J. E. and Glisson J. R. 1989. Fowl cholera in broilers. Avian Diseases, 33: 816-819.
[25] Sawada T., Borrathybay E., Kawamoto E., Koeda T. and Ohta S. 1999. Fowl cholera in Japan: disease occurrence and characteristics of Pasteurella multocida isolates. The bulletin of the Nippon Veterinary and Animal Science University, 48: 21-32.
[26] Takai H, Hayakawa Y, Shintani E, Komae H, Yoshida Y, Ide H, Abe G. and Sawada T. 1994. The first outbreak of Pasteurella multocida infection in turkeys in Japan. Journal of the Japan Veterinary Medical Association 47: 923-927.
[27] Wu, Z. J, Wu, L. Q and Cia, B. X. 1986. Comparison between primary and secondary immune responses in chickens vaccinated with fowl cholera attenuated vaccine prepared from Pasteurella multocida strain 807. Animal Husbandry and Veterinary Medicine, 18:54-56.
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