International Journal of Animal Biology
Articles Information
International Journal of Animal Biology, Vol.1, No.5, Oct. 2015, Pub. Date: Jul. 20, 2015
Specific Activities of Some Diagnostic Enzymes in Selected Tissues of Turkey (Meleagris gallopavo)
Pages: 172-175 Views: 3657 Downloads: 1441
Authors
[01] Hasan Baghshani, Department of Basic Sciences, School of V Eterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
[02] Maryam Lotfi Ghahramanloo, Department of Basic Sciences, School of V Eterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Abstract
This study was undertaken to estimate tissue specific activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatese (ALP), lactate dehydrogenase (LDH), and creatine kinase (CK) in turkey. Tissue samples of liver, kidney, heart, brain, lung, proventriculus, duodenum, and breast muscle breast were collected from freshly killed adult male turkeys. The present study results reveal the presence of all five enzymes activities in studied tissues, although in different quantities. The highest specific activity of the AST and ALT was observed in the liver followed by the kidney, with statistically significant difference b etween them. The lowest specific activity of both AST and ALT was observed in the lungs. The specific activities of ALP were significantly higher in renal and duodenal tissues of turkey. The lowest ALP levels were seen in brain, lung, proventriculus, and muscle, with no statistically significant difference among them. Highest LDH and CK activities were observed in heart and muscle and their lowest specific activities were found in duodenum and proventriculus, with no statistically significant difference between them. Since each tissue has its characteristic complement of enzymes, such information might provide valuable data for clinical and diagnostic purposes in avian v eterinary medicine. However, there is a need for further documentation of the clinical enzymology findings and also sensitivities and specificities of such tests in turkeys and other avian species.
Keywords
Enzyme, Tissue Distribution, Turkey
References
[01] Bailey,T.A., U. Wernery, J. Howl ett, A. Johnand H.Raza.1999.Diagnostic enzyme profile in Houbara Bustard tissues (Chlamydotis undulata macqueenii). Comp. Haematol. Int.9:36–42.
[02] Cornelius, C.E. 1963. Relation of body-weight to hepatic glutamic pyruvic trasaminase activity. Nature. 200:580–581.
[03] Dujovne, C.A., R. Levyand H.J. Zimmerman. 1969. The correlation b etween serum and tissue levels of enzymes in six vertebrate species--studies on lactate dehydrogenase, phosphohexoseisomerase, aldolase and malate dehydrogenase. Comp. Biochem. Physiol. 28(3): 1193-1198.
[04] Fauquier, D.A., J.A. Maz et, F.M. Gulland, T.R. Sprakerand M.M. Christopher.2008. Distribution of tissue enzymes in three species of pinnipeds. J. Zoo Wildl. Med.39(1): 1-5.
[05] Franson, J.C. 1982. Enzyme activities in plasma, liver and kidney of black ducks and mallards. J. Wildlife Dis.18: 481-485.
[06] Franson, J.C., H.C. Murrayand C. Bunck. 1985. Enzyme actives in plasma, kidney, liver, and muscle of five avian species. J. Wildlife Dis. 21(1): 33-39.
[07] Holland, K.C., A.A. Grunder, C.J. Willamsand J.S. Gavora.1980. Plasma creatine kinase as an indicator of degenerative myopathy in live turkeys. Br. Poult. Sci. 21: 161-169.
[08] Ibrahim, I., D.H. Humphreys, J.B. Stodulskiand R. Hill.1980. Plasma enzyme activities indicative of liver cell damage in laying fowl given a di et containing 20 per cent of rapeseed meal. Res. V et. Sci .28(3): 330-335.
[09] Kramer, J.W. and W.E. Hoffman. 1997. Clinical enzymology. In: Clinical Biochemistry of Domestic Animals (Ed. J.J. Kaneko, J.W. Harvey, M.L. Bruss). 5th edn. San Diego: Academic Press, pp. 303-325.
[10] Lowry, O.H., N.J. Rosebrough, A.C. Farr and R.J. Randall. 1951. Protein measurement with the Folinphenol reagent. J. Biol. Chem.193: 265–275.
[11] Lumeij, J.T. 1988. The influence of blood sample treatment, feeding and starvation on plasma glucose concentrations in racing pigeons. In: A Contribution to Clinical Investigative M ethods for Birds with Special Reference to the Racing Pigeon (Ed. J.T. Lumeij). State University, Utrecht, N etherlands, pp. 26-30.
[12] Lumeij, J.T., J.J. De Bruijne, A. Slob, J. WolfswinkelandJ.Rothuizen.1980. Enzyme activities in tissues and elimination half-lives of homologous muscle and liver enzymes in the racing pigeon (Columba Liviadomestica). Avian Pathol.17(4): 851-864.
[13] Prabakaran, R. 2003. Good practices in planning and management of integrated commercial poultry production in South Asia. FAO Animal Production and Health,159: 71-86.
[14] Szaro, R.C., M.P. Di eter, C.H. Heinzand J.R. Ferrell. 1978. Effects of chronic ingestion of south Louisiana crude oil on mallard ducklings. Environ. Res. 17: 316-436.
[15] Thrall, M., A.D. Baker, E. Lassen, D.T. Campbell, D. Denicola, M. F ettman, R. Alan and G. Weiser 2004. V eterinary Hematology and Clinical Chemistry (Ed. M.A. Thrall). Lippincott Williams and Wilkins, Philadelphia, USA, pp. 355-377.
[16] Zimmerman, H.J., C.A. Dvjowmand R. Levy 1968. The correlation of serum levels of two transaminases with tissue levels in six vertebrate species. Comp. Biochem. Physiol.25: 1081-1089.
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