International Journal of Animal Biology
Articles Information
International Journal of Animal Biology, Vol.1, No.5, Oct. 2015, Pub. Date: Aug. 3, 2015
Evaluation of Changes in Mitotic Index of Leukemia Cell Cultures in Different Time Periods
Pages: 249-252 Views: 2021 Downloads: 1098
[01] Swarupa R. Didla, Department of Human Genetics, Andhra University, Visakhapatnam, A. P., India.
[02] Jayanthi Undamatla, Department of Molecular Medicine Apollo Hospitals, Hyderabad, A. P., India.
[03] Thelagathoti Conrad Diana, Department of Zoology, Andhra University, Visakhapatnam, A. P., India.
Background: Mitotic index is a measure for the proliferation status of a cell population. The importance of the mitotic index (MI) as a prognostic factor in veterinary oncology has been emphasized recently. Materials and Method: The present data reveals that the mitotic index present in the leukemic-cell population in comparison to that of different time period over night cultures, 24 hrs cultures and 48 hrs cultures respectively. Lymphocyte culture from Leukemic blood and Bone marrow for 54 AML cases, 28 CML cases, 8 ALL cases, 2 CLL cases, 3 MDS cases and 5 other cases have been analysed by using the standard cytogenetic analytical tools. Results and Conclusion: The data derived from the three different time periods of cultures showed that relatively short period of time overnight cultures contains a large number of cells and good mitotic index. Our study shows that mitotic activity is an independent prognostic variable, possibly even more important than other biomarkers known and used in a clinical setting as indicators of risk.
[01] Killmann, S.A. Proliferative activity of blast cells in leukemia and myelofibrosis. Morphological differences between proliferating and non-proliferating blast cells. Acta med. scand. 1965, 178, 263.
[02] Japa, J. A study of the mitotic activity of normal human bone marrow. Brit. J. exp. Path. 1942, 23, 272.
[03] Preziosi R., G. Sarli and M. Paltrinieri, “Multivariate Survival Analysis of Histological Parameters and Clinical Presentation in Canine Cutaneous Mast Cell Tumours” Veterinary Research Communications, 31(3), 2007, PP. 287.
[04] Mauer, A. M., B. C. Lampkin, and E. F. Saunders. Kinetics of leukaemia cells. Proceedings of the XIth Congress of the International Society of Haematology. Plenary Sessions, Sydney, 1966, Sydney, Victor C. N. Blight, 1966, p. 42.
[05] Baserga, R. Mitotic cycle of ascites tumor cells. Arch. Path. 1963, 75, 156.
[06] Trask, B. J. Human genetics and disease: Human cytogenetics—46 chromosomes, 46 years and counting. Nature Reviews Genetics 3, 769–778 (2002) doi:10.1038/nrg905
[07] Mauer, A. M. Diurnal variation of proliferative activity in the human bone marrow. Blood 1965, 26, 1.
[08] Mauer, A. M., and V. Fisher. Comparison of the proliferative capacity of acute leukaemia cells in bone marrow and blood. Nature (Lond.) 1962, 193, 1085.
[09] Rubini, J. R., S. Keller, and E. P. Cronkite. In vitro DNA labeling of bone marrow and leukemic blood leukocytes with tritiated thymidine. 1. Physical and chemical factors which affect autoradiographic cell labeling. J. Lab. clin. Med. 1965, 66, 483.
[10] Baserga, R., and W. E. Kisieleski. Comparative study of the kinetics of cellular proliferation of normal and tumerous tissues with the use of tritiated thymidine. 1. Dilution of the label and migration of labeled cells. J. nat. Cancer Inst. 1962, 28, 331.
[11] Killmann, S. A., E. P. Cronkite, V. P. Bond, and T. M. Fliedner. Proliferation of human leukemic cells studied with tritiated thymidine in vivo. Proceedings of the VIIIth Congress of the European Society of Hematology, Vienna, 1961. Basel, S. Karger, 1962.
[12] Darbelley, N., D. Driss-Ecole, and G. Perbal. 1989. Elongation and mitotic activity of cortical cells in lentil roots grown in microgravity. Plant Physiological Biochemistry 27:341-347
[13] Driss-Ecole, D., D. Schoevaert, M. Noin, and G. Perbal. 1994. Densitometric analysis of nuclear DNA content in lentil roots grown in space. The Cell 81:59-64.
[14] Rudolph et al. (1998). "Correlation between mitotic and Ki-67 labeling indices in paraffin-embedded carcinoma specimens". Human Pathology 29: 1216–1222.
[15] Baak, J. P. A.; Gudlaugsson, E.; Skaland, I.; Guo, L. H. R.; Klos, J.; Lende, T. H.; Søiland, H. V.; Janssen, E. A. M.; Zur Hausen, A. (2008). "Proliferation is the strongest prognosticator in node-negative breast cancer: Significance, error sources, alternatives and comparison with molecular prognostic markers". Breast Cancer Research and Treatment 115 (2): 241–254.
[16] Urry et al. (2014). Campbell Biology in Focus. Pearson.
MA 02210, USA
AIS is an academia-oriented and non-commercial institute aiming at providing users with a way to quickly and easily get the academic and scientific information.
Copyright © 2014 - American Institute of Science except certain content provided by third parties.