International Journal of Animal Biology
Articles Information
International Journal of Animal Biology, Vol.1, No.5, Oct. 2015, Pub. Date: Aug. 3, 2015
Evaluation of Changes in Mitotic Index of Leukemia Cell Cultures in Different Time Periods
Pages: 249-252 Views: 2126 Downloads: 1163
Authors
[01] Swarupa R. Didla, Department of Human Genetics, Andhra University, Visakhapatnam, A. P., India.
[02] Jayanthi Undamatla, Department of Molecular Medicine Apollo Hospitals, Hyderabad, A. P., India.
[03] Thelagathoti Conrad Diana, Department of Zoology, Andhra University, Visakhapatnam, A. P., India.
Abstract
Background: Mitotic index is a measure for the proliferation status of a cell population. The importance of the mitotic index (MI) as a prognostic factor in veterinary oncology has been emphasized recently. Materials and Method: The present data reveals that the mitotic index present in the leukemic-cell population in comparison to that of different time period over night cultures, 24 hrs cultures and 48 hrs cultures respectively. Lymphocyte culture from Leukemic blood and Bone marrow for 54 AML cases, 28 CML cases, 8 ALL cases, 2 CLL cases, 3 MDS cases and 5 other cases have been analysed by using the standard cytogenetic analytical tools. Results and Conclusion: The data derived from the three different time periods of cultures showed that relatively short period of time overnight cultures contains a large number of cells and good mitotic index. Our study shows that mitotic activity is an independent prognostic variable, possibly even more important than other biomarkers known and used in a clinical setting as indicators of risk.
Keywords
MI, RPMI 1640, AML, CML, ALL, CLL
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