International Journal of Animal Biology
Articles Information
International Journal of Animal Biology, Vol.1, No.5, Oct. 2015, Pub. Date: Aug. 13, 2015
Curative Effect of Ethanolic Plant Extractives Against Beauveria bassiana Infection in Silkworm, Bombyx mori L.: Histopathological Observations on Midgut
Pages: 266-272 Views: 3060 Downloads: 1166
Authors
[01] J. A. Chavan, Department of Zoology, Shivaji University, Kolhapur, India.
[02] Y. B. Gaikwad, Department of Zoology, Shivaji University, Kolhapur, India.
[03] A. K. Chougale, Institute of Science, Fort, Mumbai, India.
[04] G. P. Bhawane, Department of Zoology, Shivaji University, Kolhapur, India.
Abstract
Beauveria bassiana is causative pathogen of white muscardine disease in silkworm Bombyx mori. Occasionally it is responsible for heavy losses to silk industry in many parts of the world. Scrutiny of herbal extracts in the disease management of silkworm, Curcuma longa, Argimone mexicana and Clerodendrum multiflorum showed antifungal activities and these plants were selected for testing their antifungal activities and their doses at 100µl/larva of 6000-ppm concentration were given on calibrated piece of leaf to the B. bassiana inoculated and starved larvae for three consecutive days. Observations on mortality and histopathology of midgut reveals that ethanolic extractive of C. longa has greater potential to check the infection of fungus B. bassiana than the extractive of A. mexicana and C. multiflorum. After making a suitable formulation and evolving farmer friendly strategy of applications, these plant products could be utilized successfully in the management of white muscardine disease in silkworm.
Keywords
Curcuma longa, Argimone mexicana, Clerodendrum multiflorum, White Muscardine
References
[01] Ahn, J. W. (1994) Cytotoxins limonoids from Melia azedarach var. Japonica. Phytochem., 36: 1493-1496.
[02] Aruga, H. (1994) Principles of Sericulture, Oxford and IBH Publishing Co PVT Ltd, Tech ED: D. Mahadevappa, Translated by Alamelu Gopal, New Delhi, India.
[03] Ayafor, J.F.; Tchuendem, M.H.K. and Nyasse, B. (1994) Novel bioactive diterpenoids from Aframomum aulacocarpos. J. Nat. Prod. 57: 917–923.
[04] Barnabas, C.G. and Nagarajan, S. (1988) Antimicrobial activity of flavonoids of some medicinal plants. Fitoterapia., 3: 508-510.
[05] Bell, J. V. (1974) Mycoses, In: Insect diseases (ED: Cartwell, GE) Vol-I, Marcel Dekker, Inc, Newyork, 185-236.
[06] Govindan, R.; Narayanswamy, T. K. and Devaiah, M. C. (1998) Principles of Silkworm Pathology, SERI Scientific Publishers: Bangalore, 31-33.
[07] Harrigan, G. G.; Ahmad, A.; Baj, N.; Glass, T. E.; Gunatilaka, A. A. L. and Kingston, D. G. I. (1993) Bioactive and other sesquiterpenoids from Porella cordeana. J. Nat. Prod. 56: 921–925.
[08] Humason, G. L. (1963) Animal tissue techniques. W. H. Freeman and Company.
[09] Iwu, M. (1999) Garcina cola: A new adaptogen with remarkable immunostimulant, antiinfective and antiinflamatory properties. A colloquium on Garcinia cola. Presented in International Conference of Ethnomedicine and Drug Discovery, Maryland, USA. 26.
[10] Iwu, M. W.; Duncan, A. R. and Okunji, C. O. (1999) New antimicrobials of plant origin. In: Janick J (editor), Perspectives on new crops and new uses. ASHS press. Alexandria. 457- 462.
[11] Janakiraman, A. T. (1961) Disease affecting the Indian silkworm races (Rerve ver soic), J. Silkworm., 13: 91-101.
[12] Jhansi, L. VVNS. (2000) Biochemical changes and diagnosis of microsporodial disease (Pebrine) of silkworm Bombyx mori L. (Lepidoptera: Bombycidae). J. Ent. Res., 24(4): 301-310.
[13] Jolly, M. S. (1987) Appropriate Sericulture Techniques, Published by Director, International Centre for Training and Research in Tropical Sericulture, Mysore, India.
[14] Kadway, M. (2009) Studies on the effect of pathogens on some vital organs of tropical tasar silkworm, Antheria mylita (Drury) (Lepidoptera: Noctuidae). Ph. D. thesis submittedto RTM, Nagpur University, Nagpur.
[15] Kawakami, K. (1973) Studies on the muscardine disease of the silkworm Bombyx mori L with special reference to the invasion of causative fungi and pathological changes in infected larva, Bull Sericul Exp Sta, 25 (5): 369-370.
[16] Khanna, N. M. (1999) Turmeric-Natures precious gift. Current Science, 76: 10: 1351-1356.
[17] Kotkar, H. M.; Mendaki, P. S.; Sadan, S. V. G. S.; Jha, S. R.; Upasani, S. M., and Maheshwari, V. L. (2001) Antimicrobial and pesticides activity of partially purified flavonoides from Annona squamossa. Pest Manag. Sci. 58: 33-37.
[18] Krishanaswami, S. (1978) New technology of silkworm rearing Central Sericulture Research and Training Institute, Mysore, Bull. 3 (2): 1-23.
[19] Krishnaswami, S. (1979) Improved method of rearing young age silkworm, C. S. R and TI Bulletin, No. 3: 1-24.
[20] Krishnaswami, S.; Narasimhanna, M. N.; Suryanarayana, S. K. and Kumararaj, S. (1987) Manual on Silkworm Rearing, Sericulture – Vol 2, FAO Agri Service Bull, 15/2, Central Silk Board, Bangalore, Rome.
[21] Kubo, I.; Muroi, H. and Himejima, M. (1993) Combination effects of antifungal nagilactones against Candida albicans and two other fungi with phenylpropanoids. J. Nat. Prod. 56: 220–226.
[22] Kumar, V.; Singh, G. H.; Basui, A. M.; Ahsam, M. M. and Datta, R. K. (1999) Germination, Penetration and inavasion of Beauveria bassiana causing white muscardine, the Italian Journal of Zoology., 66(1): 10-14.
[23] Kumar, V.; Tewari, S. K. and Awasthi, A. K. (1994) Surface ultrastructure of Beauveria bassiana infecting silkworm Bombyx mori L, Current Science., 67(7) : 546-548.
[24] Kuroyanagi, M., Arakawa, T., Hirayama, Y. and Hayashi, T., 1999. Antibacterial and antiandrogen flavonoids from Sophora flavescens. J. Nat. Prod., 62: 1595-1599.
[25] Manimeghalai, S. and Chandramohan, N. (2005) Botanicals for the management of Bacterial flacheri of silkworm, Bombyx mori L. Sericologia., 45(1): 51-58.
[26] Manimeghalai, S. and Chandramohan, N. (2006) Efficacy of botanicals against grasserie disease of silkworm, Bombyx mori L. Sericologia., 46(1): 15-23.
[27] Mathavan, S.; Sudha, P. M. and Muthu, S. P. (1991) Histological and histopathological studies on midgut epithelium of Bombyx mori larvae affected by Bacillus sphaericus. Sericologia., 31(3): 403-411.
[28] Mcmanus, J. F. A. (1948) Histological and histochemical uses of periodic acid. Stain technology. 23: 99-108.
[29] Ncube, N. S.; Afolayan, A. J. and Okoh, A. I. (2008) Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends. Afr. J. Biotchnol. 7: 1797-1806.
[30] Pringle, J. A. (1984) Report on the diseases of silkworms in India, International Books and Periodicals supply services, 24B/5, Deshbandhu gupta road, Karol Bagh, New Delhi., 57-60.
[31] Ramachandraiah, P. and Reddy, M. B. (1991) Antifungal activity of some naturally occurring flavonoids. Indian J. Microbiol., 31: 55-56.
[32] Rana, B. K.; Singh, U. P. and Taneja, V. (1999) Antifungal activity and kinetics of inhibition by essential oil isolated from leaves of Aegle marmelos. J Ethnopharmacol., 57: 29-30.
[33] Rao, K. V.; Sreeramulu, K.; Gunasekar, D. and Ramesh, D. (1993) Two new sesquiterpene lactones from Ceiba pentandra. J. Nat. Prod. 56: 2041–2045.
[34] Scheck, A. C.; Perry, k.; Hank, N. C. and Clark, W. D. (2006) Anticancer activity of extracts derived from the mature roots of Scutellaria baicalensis on human malignant brain tumor cells. Biomed. Centr. Complement. Alternat. Med. 6-27.
[35] Seetharaman, T. R.; Thirunarayanan, S. and Ganesan, T. (1995) Antifungal activity of flavonol glycosides. Geobios., 22: 87-89.
[36] Shoaf, K. A.; Chisti, M. Z. and Trag, A. R. (1993) Histopathology of the silkworm Bombyx mori L infected with Beauveria bassiana (Bals) Vuill, Indian. J. Seric., 32(2): 213-215.
[37] Silva, O.; Duarte, A.; Cabrita, J.; Pimentel, M.; Diniz, A. and Gomez, E. (1996) Antimicrobial activity of Guinea-Bissau traditional remedies. J. Ethnopharmacol. 50: 53-59.
[38] Subramani, K.; Gunasegaran, R.; Shiyamala, C. and Ganesan, T. (2004) Antifungal activity of Certain flavonol glycosides and phenolic acid from Canthium Species. Ad. Plant. Sci., 17(11): 745-748.
[39] Suresh, B.; Sriram, S.; Dhanaraj, S. A.; Elango, K. and Chinnaswamy, K. (1997) Anticandidal activity of Santolina chamaecyparissus volatile oil. J. Ethnopharmacol. 55: 151–159
[40] Suresh, G.; Narasimhan, N. S.; Masilamani, S.; Partho, P. D. and Gopalkrishnan, G. (1997) Antifungal fractions and compounds from uncrushed green leaves of Azadirachta indica. Phytoparasitica. 25(1): 33-39.
[41] Taylor, J. L. S.; Robe, T.; Mcgaw, L. J.; Jager A. k. and Van, S. J. (2001) Towards the scientific validation of traditional medicinal plants. Plant Growth Regl., 34: 23-37.
[42] Taylor, R. S. L.; Edel, F.; Manandhar, N. P. and Towers, G. H. N. (1996) Antimicrobial activities of southern Nepalese medicinal plants. J. Ethnopharmacol., 50: 97–102.
[43] Tinsley, T. W. (1975) Factors affecting virus infection of insect gut tissue. In “Invertebrate immunity” (K. Maramorosch and R. E. Shope, eds.), pp. 55-63. Academic press, New York.
[44] Ullal, S. R. and Narasimhanna, M. N. (1987) Handbook of Practical Sericulture, Central Silk Board (Ministry of Textiles-Government of India), United Mansion, 39, Mahatma Gandhi Road, Bangalore, 138-151.
[45] Widenborner, M. and Jha, H. C. (1993) Antifungal activity of flavonoids and their mixtures against different fungi occurring on gain. Pestic. Sci., 38: 347-351.
[46] Yadav, S. R. and Sardesai, M. M. (2002) Flora of Kolhapur District, Shivaji Univerity Publication., pp. 371.
[47] Yanagita, T. and Iwashita, Y. (1987) Histological observation of larvae of the silkworm, Bombyx mori orally infected with Beauveria bassiana, J. Seric. Sci, Jpn., 56(4): 285.
600 ATLANTIC AVE, BOSTON,
MA 02210, USA
+001-6179630233
AIS is an academia-oriented and non-commercial institute aiming at providing users with a way to quickly and easily get the academic and scientific information.
Copyright © 2014 - American Institute of Science except certain content provided by third parties.