International Journal of Animal Biology
Articles Information
International Journal of Animal Biology, Vol.1, No.6, Dec. 2015, Pub. Date: Sep. 26, 2015
Free-Running Eclosion Rhythm of Adult Plodia interpunctella Entrained by Either a Single Light Pulse or a Thermocycle
Pages: 281-285 Views: 2291 Downloads: 987
Authors
[01] Shigeru Kikukawa, Biological Institute, Faculty of Science, University of Toyama, Toyama, Japan.
[02] Ryou Hashizume, Biological Institute, Faculty of Science, University of Toyama, Toyama, Japan.
[03] Yuka Inoue, Biological Institute, Faculty of Science, University of Toyama, Toyama, Japan.
[04] Mina Miyabayashi, Biological Institute, Faculty of Science, University of Toyama, Toyama, Japan.
[05] Natsuko Mori, Biological Institute, Faculty of Science, University of Toyama, Toyama, Japan.
[06] Risa Sakata, Biological Institute, Faculty of Science, University of Toyama, Toyama, Japan.
[07] Yuki Takigaura, Biological Institute, Faculty of Science, University of Toyama, Toyama, Japan.
Abstract
Rhythms in physiological and behavioral activities of insects can be entrained by photoperiod and/or thermocycle (thermoperiod). The present study investigates the free-running eclosion rhythm of Plodia interpunctella Hübner (Lepidoptera: Pyralidae) by applying either a single light pulse or a thermocycle. To prevent larval diapause, all insects are maintained at 30°C and kept in constant darkness (DD) for 14 days after oviposition. Thereafter, the insects were maintained at 25°C DD. At day 27 after oviposition, the insects are subjected to a single light pulse (2-16 h). Average time from the light-off signal to the first peak of eclosion rhythm in DD is c. 17.5 h, indicating that the insects interpret the ‘light-off’ signal as a Zeitgeber to entrain the rhythm at 25°C, since the rhythm normally free-runs for a period of c. 23 h. A single light pulse in otherwise DD can entrain the adult eclosion rhythm in this species at 25°C. In contrast, the rhythm can be entrained by applying a thermocycle consisting of 30°C thermophase (12 h) and 20°C cryophase (12 h) regardless of background illumination (DD or constant illumination (LL)). The insects are exposed to the thermocycle in DD or LL from days 15 to 28 after oviposition and subsequently maintained at 25°C in DD or LL. The rhythm is observed to free-run for a period of c. 22 h. Thus, the adult eclosion rhythm in this species can be entrained by either a light pulse or a thermocycle. However, it is unclear whether the photoperiodic and thermoperiodic time-keeping mechanisms are controlled by the same physiological processes.
Keywords
Adult Eclosion, Free-Running Rhythm, Plodia Interpunctella, Light Pulse, Thermoperiod
References
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