International Journal of Animal Biology
Articles Information
International Journal of Animal Biology, Vol.1, No.6, Dec. 2015, Pub. Date: Dec. 14, 2015
Isolation and Identification of Equine Influenza H3N8 Virus from Clinical Cases in Khartoum State, Sudan
Pages: 298-301 Views: 1265 Downloads: 889
Authors
[01] Wegdan H. A., Veterinary Research Institute, Khartoum, Sudan.
[02] Intisar K. Saeed., Veterinary Research Institute, Khartoum, Sudan.
[03] Shaza M. M., Veterinary Research Institute, Khartoum, Sudan.
[04] Algezoli O. A., Veterinary Research Institute, Khartoum, Sudan.
[05] Ballal A., Veterinary Research Institute, Khartoum, Sudan.
[06] Ihsan H. A., Veterinary Research Institute, Khartoum, Sudan.
[07] Sahar M. E., Veterinary Research Institute, Khartoum, Sudan.
[08] Baraa A. M., Veterinary Research Institute, Khartoum, Sudan.
[09] Ahmed O. A., Veterinary Research Institute, Khartoum, Sudan.
[10] Ali Y. H., Veterinary Research Institute, Khartoum, Sudan.
Abstract
In June 2013, a severe respiratory disease with high morbidity 75% (75/100) and low mortality (2%) occurred at the Khartoum Horse Racing Stable (KHRS); nasal discharge “serous to mucopurulent”, dry hacking cough and pyrexia were the main clinical signs observed. The one animal subjected to Post mortem examination showed, congestion in lung and liver and myocarditis. During the course of the disease which lasted about one week. 20 out of 23 horses (87%) at Halfaya Stable (Khartoum north), showed clinical signs similar to that of Khartoum Stable, but none of the horses died. Equine influenza H3N8 virus was isolated from lung and nasal swab samples inoculated into allantoic cavity of 10 day embryonating chicken eggs. The virus was confirmed serologically by HA & HI tests and by one step RT-PCR. The results of the study confirmed the presence of influenza a virus in horses in Khartoum state as well as the isolation of the virus for the first time in the country from clinical cases.
Keywords
Equine Influenza, Clinical Sign, One - Step RT - PCR
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