International Journal of Animal Biology
Articles Information
International Journal of Animal Biology, Vol.1, No.3, Jun. 2015, Pub. Date: May 18, 2015
Transplantation of Goat Spermatogonial Stem Cells into the Mouse Rete Testis
Pages: 61-68 Views: 3251 Downloads: 1579
Authors
[01] Mohammad Sadra Shirazi, Reproductive Biotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran.
[02] Banafsheh Heidari, Reproductive Biotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran.
[03] Mohammad Mehdi Naderi, Reproductive Biotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran.
[04] Bahareh Behzadi, Reproductive Biotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran.
[05] Ali Sarvari, Reproductive Biotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran.
[06] Sara Borjian-Boroujeni, Reproductive Biotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran.
[07] Morteza Farab, Reproductive Biotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran.
[08] Abolfazl Shirazi, Reproductive Biotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran;Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran.
Abstract
Assisted reproductive techniques involving isolation, culture, and transplantation of spermatogonial stem cells offer unique approach to manipulate the male germline. The application of these techniques in farm animals has been the subject of an increasing number of studies, mostly because of its potential as an alternative strategy in producing transgenic livestock with higher efficiency and less time and capital requirement than the current methods. The aim of this study was to assess the colonization and differentiation potentials of enriched goat spermatogonia into the mouse testes. Moreover, because stem cells may need to be preserved for several years before re-introduction to the recipient testes, we developed the efficient cryopreservation technique for type A spermatogonoa. The enzymatically isolated SSCs obtained from one month old goats’ testes were enriched by using discontinuous percoll density, and followed by cryopreservation protocol. After xenotransplantation of prepared goat testicular cells into the mouse rete testis, the proliferative activity and stemness potential of SSCs were evaluated and compared with in vitro culture condition. We demonstrated that the viability of testicular cells after cryopreservation was significantly lower than fresh cells, although these cells had normal structural and functional characteristics (P <0.001). Donor goat spermatogonia were able to survive and colonize in depleted recipient’s testis at 80 days after transplantation, but later stages of donor-derived spermatogenesis were not observed at this time. Although cross-species spermatogonial transplantation did not have the envisioned immediate practical application, it nonetheless provides a bioassay for stem cell potential of germ cells isolated from other species.
Keywords
Cryopreservation, Goat, Mouse, PGP9.5, Spermatogonial Stem Cells, Transplantation
References
[01] Abrishami M., Anzar M., Yang Y. and Honaramooz A. 2010. Cryopreservation of immature porcine testis tissue to maintain its developmental potential after xenografting into recipient mice. Theriogenology, 73: 86-96.
[02] Brinster R.L. and Zimmermann J.W. 1994. Spermatogenesis following male germ-cell transplantation. P.N.A.S. USA, 91: 11298-11302.
[03] Creemers L.B., Meng X., den Ouden K., van Pelt AM., Izadyar F., Santoro M., Sariola H. and de Rooij DG. 2002. Transplantation of germ cells from glial cell line-derived neurotrophic factor-overexpressing mice to host testes depleted of endogenous spermatogenesis by fractionated irradiation. Biol. Reprod., 66: 1579-84.
[04] Dirami G., Ravindranath N., Pursel V. and Dym M. 1999. Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM. Biol. Reprod., 61: 225-230.
[05] Dobrinski I., Avarbock M.R. and Brinster R.L. 1999. Transplantation of germ cells from rabbits and dogs into mouse testes. Biol. Reprod., 61: 1331-1339.
[06] Dobrinski I., Avarbock M.R. and Brinster R.L. 2000. Germ cell transplantation from large domestic animals into mouse testes. Mol Reprod. Dev., 57: 270-279.
[07] Dobrinski I. 2006. Transplantation of germ cells and testis tissue to study mammalian spermatogenesis. Anim. Reprod., 3(2): 135-145.
[08] Fujita K., Tsujimura A., Miyagawa Y., Kiuchi H., Matsuoka Y., Takao T., Takada S., Nonomura N. and Okuyama A. 2006. Isolation of germ cells from leukemia and lymphoma cells in a human in vitro model: potential clinical application for restoring human fertility after anticancer therapy. Cancer Res., 66: 11166-71.
[09] Heidari B., Gifani M., Shirazi A, Zarnani A.H., Baradaran B., Naderi M.M., Behzadi B., Borjian-Boroujeni S., Sarvari A., Lakpour N. and Akhondi M.M. 2014. Enrichment of undifferentiated type A spermatogonia from goat testis using discontinuous percoll density gradient and differential plating. AJMB., 6: 94-103.
[10] Honaramooz A. and Yang Y. 2011. Recent Advances in Application of Male Germ Cell Transplantation in Farm Animals. Vet Med Int. 2011, pii: 657860. doi:10.4061/2011/657860.
[11] Izadyar F., Spierenberg G., Creemers L., den Ouden K. and de Rooij D.G. 2002. Isolation and purification of type A spermatogonia from the bovine testis. Reproduction, 124: 85-94.
[12] Jiang F.X. 2001. Male germ cell transplantation: promise and problems Reproduction. Fertil. Dev., 13: 609-614.
[13] Kanatsu-Shinohara M., Ogonuki N., Inoue K., Ogura A., Toyokuni S. and Shinohara T. 2003. Restoration of fertility in infertile mice by transplantation of cryopreserved male germline stem cells. Human Reprod., 18(12): 2660-2667.
[14] Kaul G., Kumar Sh. and Kumari S. 2012. Enrichment of CD9+ spermatogonial stem cells from goat (Capra aegagrus hircus) testis using magnetic microbeads. Stem Cell Discov., 2: 92-99.
[15] Kim Y., Turner D., Nelson J., Dobrinski I., McEntee M. and Travis A.J. 2008. Production of donor-derived sperm after spermatogonial stem cell transplantation in the dog. Reproduction, 136(6): 823-831.
[16] Kim Y., Selvaraj V., Dobrinski I., Lee H., McEntee M.C. and Travis A.J. 2006. Recipient preparation and mixed germ cell isolation for spermatogonial stem cell transplantation in domestic cats. J. Androl., 27: 248-256.
[17] Liu S., Tang Z., Xiong T. and Tang W. 2011. Isolation and characterization of human spermatogonial stem cells. Reprod. Biol. Endocrin., 9: 141-150.
[18] Marret C. and Durand P. 2000. Culture of porcine spermatogonia: Effects of purification of the germ cells, extracellular matrix, and fetal calf serum on their survival and multiplication. J. Reprod. Dev., 40: 305-319.
[19] Nagano A., Avarbock M.R. and Brinster R.L. 1999. Pattern and kinetics of mouse donor spermatogonial stem cell colonization in recipient testes. Biol. Reprod., 60: 1429-36.
[20] Nagano M., Mc Carrey J.R. and Brinster R.L. 2001. Primate spermatogonial stem cells colonize mouse testes. Biol. Reprod., 64: 1409-1416.
[21] Nagano M., Patrizio P. and Brinster R.L. 2002. Long-term survival of human spermatogonial stem cells in mouse testes. Fertil. Steril., 78: 1225-1233.
[22] Ogawa T., Arechaga J.M., Avarbock M.R. and Brinster R.L. 1997. Transplantation of testis germinal cells into mouse seminiferous tubules. Int. J. Dev. Biol. , 41: 111-122.
[23] Ogawa T., Dobrinski I., Avarbock M.R. and Brinster R.L. 1999. Xenogeneic spermatogenesis following transplantation of hamster germ cells to mouse testes. Biol. Reprod., 60: 515-521.
[24] Schlatt S., Rosiepen G., Weinbauer G.F., Rolf C., Brook P.F. and Nieschlag E. 1999. Germ cell transfer into rat, bovine, monkey and human testes. Human Reprod., 14: 144-50.
[25] Shinohara T., Avarbock M.R. and Brinster R.L. 2000. Functional analysis of spermatogonial stem cells in steel and cryptorchid infertile mouse models. Dev. Biol., 220: 401-11.
[26] Shinohara T., Orwig K.E., Avarbock M.R. and Brinster R.L. 2002. Germ line stem cell competition in postnatal mouse testes. Biol. Reprod., 66: 1491-7.
[27] Sugiyama N., Obinata M. and Matsui Y. 2001. BCL-2 inhibits apoptosis of spermatogonia and growth of spermatogenic stem cells in a cell-intrinsic manner. Mol. Reprod. Dev., 58(1): 30-38.
[28] Tang L., Rodriguez-Sosa J.R. and Dobrinski I. 2012. Germ cell transplantation and testis tissue xenografting in mice. J. Vis. Exp., 6(60), pii: 3545. doi:10.3791/3545.
[29] van Pelt A.M., Morena A.R., van Dissel-Emiliani F.M., Boitani C., Gaemers I.C., de Rooij D.G. and Stefanini M. 1996. Isolation of the synchronized A spermatogonia from adult vitamin A-deficient rat testes. Biol .Reprod., 55: 439-444.
[30] Wang D.Z., Zhou X.H., Yuan Y.L., Yuan Y.L. and Zheng X.M. 2010. Optimal dose of busulfan for depleting testicular germ cells of recipient mice before spermatogonial transplantation. A. J. Andro., 12: 263-270.
600 ATLANTIC AVE, BOSTON,
MA 02210, USA
+001-6179630233
AIS is an academia-oriented and non-commercial institute aiming at providing users with a way to quickly and easily get the academic and scientific information.
Copyright © 2014 - American Institute of Science except certain content provided by third parties.