International Journal of Animal Biology
Articles Information
International Journal of Animal Biology, Vol.1, No.3, Jun. 2015, Pub. Date: Jun. 2, 2015
Avian Influenza Resistant Gene (Mx) and Its Diversity in Chicken and Duck
Pages: 78-85 Views: 2772 Downloads: 1269
Authors
[01] Minakshi Nag, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.
[02] Md. Mostafizer Rahman, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.
[03] Anjuman Ara Bhuyan, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka, Bangladesh.
[04] Mridha Md. Kamal Hossain, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka, Bangladesh.
[05] Md. Abdul Alim, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka, Bangladesh.
[06] Md. Saidul Islam, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka, Bangladesh.
[07] Mir Rowsan Akter, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.
[08] Mahmudul Hasan, Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh.
[09] Jahangir Alam, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka, Bangladesh.
Abstract
Poultry sector has been facing threat of highly pathogenic avian influenza (HPAI) since 2007 in Bangladesh. The chicken Mx protein has been reported to exhibit antiviral activity against influenza virus. Therefore, targeting Mx gene may be an approach for development of AI resistant poultry. However, there is scarcity of information about the Mx gene and its diversity in chicken and duck in Bangladesh. To enumerate Mx gene and its diversity a total of 60 blood samples were collected from six chicken groups (Rhode Island Red, White Leghorn, White Rock, Barred Plymouth Rock, Necked Neck & Hilly) and four duck groups (Pekin, Rupali, Nageshwari & Common Deshi) from Bangladesh Livestock Research Institute, Nageshwary, Kurigram district and Natore Sadar, Natore district. Two sets of primers were used. Of them one set for Mx gene detection by PCR and another set for Mx gene detection and its diversity analysis by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) using RsaI and SspI restriction enzymes. The Mx gene detection with one set primer was found to vary from 60.0-100% (average 77.8%) in chicken and 0-40% (average 43.6) in duck. However, with another set of primer detection rate was 40-100 and 66.7-100% in chicken and duck, respectively. PCR-RFLP analysis of 27 samples revealed that tested samples contain homozygous resistant (R/R), homozygous sensitive (S/S) and heterozygous (R/S) Mx allelic gene. In chicken the R/R, S/S and R/S Mx allelic gene was 38.9, 33.3 and 27.0% respectively; while in duck it was 11.1, 66.7 and 22.2%, respectively. Ducks were found more sensitive then tested chicken. Mx gene diversity was found to exist not only among the groups but also within the group of chicken and duck. To the best of our knowledge this is the first report on Mx gene study in Bangladesh covering both chicken and duck.
Keywords
Mx Gene, Diversity, Chicken, Duck, Avian Influenza
References
[01] Abdelwhab, E. M., and H.M. Hafez. 2012. Insight into alternative approaches for control of avian influenza in poultry, with emphasis on highly pathogenic H5N1. Viruses, 4: 3179-3208.
[02] Aebi, M., J. Fah, N. Hurt, C.E. Samuel, D. Thomis, L. Bazzigher, J. Pavlovic, O. Haller and P. Staeheli. 1989. cDNA structures and regulation of two interferon-induced human Mx proteins. Mol. Cell. Biol.,9:5062-5072.
[03] Baise, E., G.G. Pire, M.I. Leroy, J.I. Garardin, N. Goris, K.D. Clercq, P. Kerkhofs and D. Desmecht. 2004. Conditional expression of type I interferon-induced bovine Mx1GTPase in a stable transgenic vero cell line interferes with replication of vesicular stomatitis virus. J. of Interferon & Cytokine Res., 24:513-521.
[04] Bazzigher, L., A. Schwarz and P. Staeheli. 1993. No enhanced influenza virus resistance of murine and avian cells expressing cloned duck Mx protein. Virol., 195:100-112.
[05] Benfield, C.T.O., J.W. Lyall, G. Kochs and L.S. Tiley. 2008. Asparagine 631 variants of the chicken Mx protein do not inhibit influenza virus replication in primary chicken embryo fibroblasts or in vitro surrogate assays. J. Virol., 82:7533-7539.
[06] Bernasconi, D., U. Schultz and P. Staeheli. 1995. The interferon-induced Mx protein of chickens lacks antiviral activity. J. of Interferon & Cytokine Res., 15: 47-53.
[07] Bhuiyan, A., M.S.A. Bhuiyan and G.K. Deb. 2005. Indigenous chicken genetic resources in Bangladesh: Current status and future outlook. Anim. Genet. Resources Informatio., 36: 73-84.
[08] Bhuiyan, M.S.A., S. Chen, S. Faruque A. Bhuiyan and A. Beja-Pereira. 2013. Genetic diversity and maternal origin of Bangladeshi chicken. Mol.Boil. Rep,40: 4123-4128.
[09] FAO. 2014. Update on the continuous spread and expansion of H5N1 highly pathogenic avian influenza: Clade 2.3.2.1 in Asia (2010–2012), FOCUS on, No. 7, January 2014. Rome.
[10] Garber, E.A., C.I. Rosenblum, H.T. Chute, L.M. Scheidel and H. Chen. 1991. Avian retroviral expression of luciferase. Virol., 185: 652-660.
[11] Giasuddin, M., M.E. Haque, A.H.M. Kamal, M.R. Islam, A. Jahangir, E.H. Chowdhury, M.J.F.A. Taimur and M.H. Rahman. 2013. Outbreak evaluation of highly pathogenic avian influenza in Bangladesh. Bang. J. Lives. Res. 19 (1-2): 41-45
[12] Haller, O., M. Frese, D. Rost, P.A. Nuttall and G. Kochs. 1995. Tick-borne thogoto virus infection in mice is inhibited by the Orthomyxovirus resistance gene product Mx1.J Virol., 69:2596-2601.
[13] Haller, O., S. Stertz and G. Kochs. 2007. The MxGTPase family of interferon-induced antiviral proteins. Microbes and Infection, 9:1636-1643.
[14] Horisberger, M.A. 1995. Interferons, Mx genes, and resistance to influenza virus. American J. Respir. Crit. care Med., 152:S67.
[15] ICDDR,b. 2013. Unusual waterfowl mortality due to highly pathogenic avian influenza A (H5N1) virus in Netrokona, Bangladesh 2011. Health and Science Bulletin.11 (1). March 2013.
[16] Jahangir, A., H. Mahmudul, H. Kamrul, M. Giasuddin, M.R. Hafizur, A. Shahin and M.J.F.A. Taimur. 2015. Diversity of Myxovirus resistance gene (Mx gene) in chicken. Proceedings of the 9th International Poultry show and Seminar of World’s Poultry Science Association- Bangladesh Branch held on 19-21 February 2015. pp. 165-172
[17] Janzen, C., G. Kochs and O. Haller. 2000. A monomeric GTPase-negative MxA mutant with antiviral activity. J. Virol.,74:8202-8206.
[18] Jin, H.K., A. Takada, Y. Kon, O. Haller and T. Watanabe. 1999. Identification of the murine Mx2 gene: interferon-induced expression of the Mx2 protein from the feral mouse gene confers resistance to vesicular stomatitis virus. J. Virol.,73:4925-4930.
[19] Ko, J.H., H.K. Jin, A. Asano, A. Takada, A. Ninomiya, H. Kida, H. Hokiyama, M. Ohara, M. Tsuzuki and M. Nishibori. 2002. Polymorphisms and the differential antiviral activity of the chicken Mx gene. Genome Res,12:595-601.
[20] Landis, H., A. Simon-Jadicke, A. Klati, C. Di Paolo, J.J.R. Schnorr, S. Schneider-Schaulies, H. P. Hefti and J. Pavlovic. 1998. Human MxA protein confers resistance to Semliki Forest virus and inhibits the amplification of a Semliki Forest virus-based replicon in the absence of viral structural proteins. J. Virol.,72:1516-1522.
[21] Lee, S.H. and S.M. Vidal. 2002. Functional diversity of Mx proteins: variations on a theme of host resistance to infection. Genome Res.,12:527-530.
[22] Li, X.Y., L.J. Qu, Z.C. Hou, J.F. Yao, G.Y. Xu and N. Yang. 2007. Genomic structure and diversity of the chicken Mx gene. Poultry Scienc., 86:786-789.
[23] Livant, E.J., S. Avendano, S. McLeod, X. Ye, S.J. Lamont, J.C.M. Dekkers and S.J. Ewald. 2007. MX1 exon 13 polymorphisms in broiler breeder chickens and associations with commercial traits. Anim. Genet., 38: 177-179
[24] Meier, E., G. Kunz, O. Haller and H. Arnheiter. 1990. Activity of rat Mx proteins against a rhabdovirus. J. Virol.,64:6263-6269.
[25] Muller, U., U. Steinhoff, L.F. Reis, S. Hemmi, J. Pavlovic, R.M. Zinkernagel, and M. Aguet. 1994. Functional role of type I and type II interferons in antiviral defence. Sci.,264:1918-1921.
[26] Pavlovic, J., O. Haller and P. Staeheli. 1992. Human and mouse Mx proteins inhibit different steps of the influenza virus multiplication cycle. J. Virol.,66:2564-2569.
[27] Rothman, J.H., C.K. Raymond, T. Gilbert, P.J. O'Hara and T.H. Stevens. 1990. A putative GTP binding protein homologous to interferon-inducible Mx proteins performs an essential function in yeast protein sorting. Cell, 61:1063-1074.
[28] Sartika, T., S. Sulandari, and M.S.A. Zein. 2011. Selection of Mx gene genotype as genetic marker for Avian Influenza resistance in Indonesian native chicken. Pp. S37. BMC proceedings. BioMed Central Ltd.
[29] Seyama, T., J.H. Ko, M. Ohe, N. Sasaoka, A. Okada, H. Gomi, A. Yoneda, J. Ueda, M. Nishibori and S. Okamoto. 2006. Population research of genetic polymorphism at amino acid position 631 in chicken Mx protein with differential antiviral activity. Biochem. Genet,44:432-443.
[30] Sironi, L., P. Ramelli, J.L. Williams and P. Mariani. 2010. PCR-RFLP genotyping protocol for chicken Mx gene G/A polymorphism associated with the S631N mutation. Genet. Mol. Res.,9:1104-1108.
[31] Subbarao, K., A. Klimov, J. Katz, H. Regnery, W. Lim, H. Hall, M. Perdue, D. Swayne, C. Bender, J. Huang, M. Hemphill, T. Rowe, M. Shaw, X. Xu, K. Fukuda and N. Cox. 1998. Characterization of an avian influenza A (H5N1) virus isolated from a child with a fatal respiratory illness. Science. 279, 393–396.
[32] Sulandari, S., M. SyamsulArifinZein, D. Astuti and T. Sartika. 2009. Genetic polymorphisms of the chicken antiviral Mx gene in a variety of Indonesian indigenous chicken breeds. J. Veteriner., 10: 50-56.
[33] Schneider-Schaulies, S., J.R. Schneider-Schaulies, A. Schuster, M. Bayer, J. Pavlovic and V. TerMeulen. 1994. Cell type-specific MxA-mediated inhibition of measles virus transcription in human brain cells. J. Virol., 68:6910-6917.
[34] Schwemmle, M., K.C. Weining, M.F. Richter, B. Schumacher and P. Staeheli. 1995. Vesicular stomatitis virus transcription inhibited by purified MxA protein. Virol., 206:545-554.
[35] Zurcher, T., J. Pavlovic and P. Staeheli. 1992. Mechanism of human MxA protein action: variants with changed antiviral properties. Embo J.,11(4):1657-61.
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