International Journal of Animal Biology
Articles Information
International Journal of Animal Biology, Vol.1, No.3, Jun. 2015, Pub. Date: Jun. 2, 2015
Avian Influenza Resistant Gene (Mx) and Its Diversity in Chicken and Duck
Pages: 78-85 Views: 2772 Downloads: 1269
[01] Minakshi Nag, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.
[02] Md. Mostafizer Rahman, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.
[03] Anjuman Ara Bhuyan, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka, Bangladesh.
[04] Mridha Md. Kamal Hossain, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka, Bangladesh.
[05] Md. Abdul Alim, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka, Bangladesh.
[06] Md. Saidul Islam, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka, Bangladesh.
[07] Mir Rowsan Akter, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.
[08] Mahmudul Hasan, Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh.
[09] Jahangir Alam, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka, Bangladesh.
Poultry sector has been facing threat of highly pathogenic avian influenza (HPAI) since 2007 in Bangladesh. The chicken Mx protein has been reported to exhibit antiviral activity against influenza virus. Therefore, targeting Mx gene may be an approach for development of AI resistant poultry. However, there is scarcity of information about the Mx gene and its diversity in chicken and duck in Bangladesh. To enumerate Mx gene and its diversity a total of 60 blood samples were collected from six chicken groups (Rhode Island Red, White Leghorn, White Rock, Barred Plymouth Rock, Necked Neck & Hilly) and four duck groups (Pekin, Rupali, Nageshwari & Common Deshi) from Bangladesh Livestock Research Institute, Nageshwary, Kurigram district and Natore Sadar, Natore district. Two sets of primers were used. Of them one set for Mx gene detection by PCR and another set for Mx gene detection and its diversity analysis by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) using RsaI and SspI restriction enzymes. The Mx gene detection with one set primer was found to vary from 60.0-100% (average 77.8%) in chicken and 0-40% (average 43.6) in duck. However, with another set of primer detection rate was 40-100 and 66.7-100% in chicken and duck, respectively. PCR-RFLP analysis of 27 samples revealed that tested samples contain homozygous resistant (R/R), homozygous sensitive (S/S) and heterozygous (R/S) Mx allelic gene. In chicken the R/R, S/S and R/S Mx allelic gene was 38.9, 33.3 and 27.0% respectively; while in duck it was 11.1, 66.7 and 22.2%, respectively. Ducks were found more sensitive then tested chicken. Mx gene diversity was found to exist not only among the groups but also within the group of chicken and duck. To the best of our knowledge this is the first report on Mx gene study in Bangladesh covering both chicken and duck.
Mx Gene, Diversity, Chicken, Duck, Avian Influenza
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