[01] Akin-Osanaiye B. C., Department of Microbiology, Faculty of Science, University of Abuja, Abuja, Nigeria. [02] Nok A. J., Department of Biohemistry, Faculty of Science, Ahmadu Bello University, Zaria, Nigeria. [03] Amlabu E., Department of Biohemistry, Faculty of Science, Kogi State University, Anyigba, Nigeria;Department of Biohemistry, Faculty of Science, Kaduna State University, Kaduna, Nigeria. [04] E. Haruna, Department of Biohemistry, Faculty of Science, Kaduna State University, Kaduna, Nigeria.

Malaria is the most important of the parasitic disease of humans, and its neurological complications cerebral malaria is arguably one of the most common non-traumatic encephalopathies, and it is becoming more difficult to manage particularly in areas of multi-drug resistance. The clinical symptoms include anaemia and cerebral haemorrhage. Hence, This study was designed to assess changes in serum haematological parameters in the Plasmodium berghei infected albino mice treated with neem extracts. Albino mice free from infection were experimentally infected with Plasmodium berghei. The animals showed detectable parasitemia on day 4 post-infection with about 30 % of parasitemia recorded before death of animal. Packed cell volume (PCV) level decreased with increase in the post infection days. The PCV values in all the infected treated experimental animals were significantly different (p≤0.05) from the infected untreated animals. Other heamatological parameters which include HB and WBC decreased with increased parasitemia. A decrease in packed cell volume an index of anaemia was observed in the Plasmodium berghei infected mice. Clinical signs observed during the course of treatment ranged from diarrhea, anaemia, loss of appetite, loss of weight to cerebral hemorrhage and splenomegaly.

Malaria, Parasitemia, Heamatological Parameters, Plasmodium berghei, Anaemia

[01] Aikawa, M. (1988). Human Cerebral Malaria AMJ. Trop. Med. Hyg. 39: 3-10. [02] Akin-Osanaiye BC, Nok AJ, Ibrahim S, Inuwa HM, Onyike E, Amlabu E and E. Haruna (2013). antimalarial Effect of Neem Leaf and Neem Stem Bark Extracts on Plasmodium berghei Infected Mice in the Pathology and Treatment of Malaria. Int. J Res Biochem. Biophy. 3(1): [03] Ayodele T. (1979) Studies on Azadirachta indica in malaria. 4th OAU inter African symposium Abidjan, Ivory Coast. [04] Bradley DJ, Neewbold CI, Warrel DA (1987). Anaemia. Oxford textbook of medicine. Vol. 1.pp. 5474-5502. ELBS, Second edition. Great Britain. [05] Breman T. G, Alilo M.S and Millis A. (2004): Conquering the intolerable burden of Malaria. What’s new? what’s needed? A summary. AMJ. Trop. Med. Hyg. II: 1-15 [06] Carter rand Diggs C.L (1977): Plasmodia of rodents in parasitic protozoa, Vol. III. Pp 359-465. [07] Dacie JV, and Lewis SM. eds. Practical Hamatology. 8th ed. Churchil Livingstone. 1995:287-296. [08] Daily, JP (2006). Antimalarial Drug Theraapy. The Role of Parasite Biology and Drug resistance. J Clin Pharmacol. 46: 1487-1497. [09] David, A. F., Philip, J.R, Simon, L.C, Reto B, and Solomon N (2004). Antimalarial drug discovery: efficacy models for compound screening. Nature reviews. 16: 522-528. [10] Dawit D, Eyassu M, Asfaw D, Dawit A, Kelbessa U, Wallelign, M, Daniel M. Ashenafi A and Yared M. (2006). In vivo anti-malarial activity of hydro alcoholic extracts from Asparagus africanus Lam. in mice infected with plasmodium berglei. Ethoip J. Health Dev. 20(2):112-118. [11] Esievo, KAN, Saror DI (1992) Determination of packed cell volume and total protein concentration. In manual of veterinary clinical pathology (1st edtn). Ahmadu Brllo University Press. Zaria, Nigeria. [12] Esievo, KAN., Saror, D I. Ilemobade, A A., Hallaway, MH (1982). Variation in erythrocyte surface and free serum sialic sacid concentrations during experimental T.vivax infection in cattle. Res. Vet Sci. 32, 1-5. [13] Felsher, B. F. and Redeker, A. G. (1966): Effect of chloroquine on hepatic uroporphyrin metabolism in patients with porphyria cutanea tarda. Medicine (Baltimore); 45:575-583. [14] Greenwood BM, Bojang K, Whitty CJ, Targett GA (2005). "Malaria". Lancet 365: 1487-1498. [15] Janse, CJ, and Waters, AP. (2006). The Plasmodium berghei Reasearch Model of Malaria. Medical center. Leiden University. [16] Kakkilaya, BS (2006). Malaria Life cycle. http/www. malariasite.com. [17] Kota A. K.; Sashi S. and Phanithi P. B (2006). Studies on the glycoprotein modification in erythrocyte membrane during experimental cerebral malaria. Experimental Parasitol. 114(3): 173-179. [18] Kronfeld DS: Medway W. Blood chemistry. In: Medway W., Prier J. E., Wilkinson J. S. (12th eds.): Textbook of Veterinary Clinical Pathology 1969. [19] Marsh, k., Forster, D. and Waruiru, C. (1995). Indicators of Life threatening malaria in African children. N Engl J Med. 332:1399-1404. [20] Murphy, SC, and Breman, JG. (2001). Gaps in the childhood malaria burden in Africa:Cerebral malaria, neurological sequelae, anemia, respiratory distress, hypoglycemia, and complications of pregnancy. Am J Trop Med Hyg 64(suppl 1-2):57-67. [21] Newton RJC, Hien TT and White N (2000). Neurological Aspects of Tropical Diseases: Cerebral malaria. J. Neurol. Neurosurg. Psychiatry.69:433-441. [22] Ononiwu K. I., Useh N. M. (2005). Plasmodium berghei produce neuraminidase. Journal of Thrombosis and Haemostasis; Volume 5, Supplement 1:074P. [23] Osonuga, IO, Osonuga, OA, Osonuga, A, Onadeko, AA and AA Osonuga (2012). Effect of artemether on hematological parameters of healthy and uninfected adult Wistar rats. Asian Pac J Trop Biomed. 2(6): 493–495. [24] Peter I. T and Anatoli V.K. (1998): the current global malaria situation. Malaria parasite biology, pathogenesis, and protection ASM press. W.D.C Pp 11-12. [25] Rogerson, S. (2006). What is the Relationship between Heptoglobin, Malaria, and Anaemia? PLOS medicine/www.plosmedicine.org.vol3(5):593-594. [26] Winstanley PA (2000). Chemotherapy for falciparum malaria: The armoury, the problems and the prospects. Parasitol. Today. 16:146-153. [27] Wright, C. W (2005). Plant Derived Anti-malaria agents: New Leads and Challenges. phytochemistry reviews. 4: 55-61. [28] Yakubu, M. T., Akanji, M. A. and Oladiji, A. T. (2007) "Haematological evaluation in male albino rats following chronic administration of aqueous extract of Fadogia agrestis stem". Pharmacognosy Magazine; 3(1): 34-38.