International Journal of Chemical and Biomolecular Science
Articles Information
International Journal of Chemical and Biomolecular Science, Vol.1, No.3, Oct. 2015, Pub. Date: Aug. 23, 2015
Evaluation Using Fourier Transformed-Infrared Spectroscopy (FT-IR) of Biodegradation by the Strain Trichoderma Viride M5-2 from the Cell Walls of Sugarcane (Saccharum Officinarum Lin) Bagasse Pretreated
Pages: 134-140 Views: 2427 Downloads: 1141
Authors
[01] Elaine Valiño, Institute of Animal Science, Physiology Department, Mayabeque, Cuba.
[02] A. Elías, Institute of Animal Science, Physiology Department, Mayabeque, Cuba.
[03] Marlen Rodríguez, Institute of Materials and Reactive (IMRE), University of Havana, La Habana, Cuba.
[04] Nereyda Albelo, Institute of Animal Science, Physiology Department, Mayabeque, Cuba.
Abstract
The objective was to prove the molecular change produced in the sugarcane bagasse with pretreated calcium hydroxide and fermented with Trichoderma viride M5-2 strain by means of Fourier Transform Infrared Spectroscopy (FTIR).The fungi fermentation was conducted in a solid state bioreactor. For the spectroscopy analysis by FTIR, the samples were taken from sugarcane bagasse, sugarcane bagasse hydrolyzed with Ca (OH) 2 and fermented at a depth of 10 cm in the bioreactor at 96 h, when the highest cellulolytic activity of exo ß1-4 glucanase (2.8 UI/ml) was reached.The spectrum of the intact sugar cane bagasse and the bagasse hydrolyzed with Ca (OH) 2 differed in the range 2000-1400±20 cm-1 of transmittance. Moreover, differences were found in the sugarcane layer during the fermentation. In the fermented sample spectrum, new bands were visualized in the range 774.30±20 cm-1 and 1670-1780±20 cm-1, when obtaining increases in carbonyl, carboxyl and phenolic groups derived from the breaking of the subunits of lignin and structural carbohydrate. The biodegradative effect of the Trichoderma viride M5-2 strain sin the pretreated sugar cane bagasse with Ca (OH) 2 was proved by means of the FTIR technique.The production of the endo and exo β 1-4 glucanase enzyme treated with calcium hydroxide bagasse improved. The cellulases produced during the conversion from cellulose to glucose by the fungal mutant strain depend directly on the treatment.
Keywords
Infrared Spectroscopy, Trichodema viride, Sugar Cane Bagasse, Solid State Fermentation
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