International Journal of Chemical and Biomolecular Science
Articles Information
International Journal of Chemical and Biomolecular Science, Vol.1, No.3, Oct. 2015, Pub. Date: Aug. 12, 2015
Application of Microsatellite SSR Markers in a Number of Pomegranate (Punica granatum L.) Cultivars in Kurdistan Region/Duhok Province
Pages: 117-122 Views: 3868 Downloads: 1720
Authors
[01] Dalal Y. Sinjare, Scientific Research Center, Duhok University, Duhok, Iraq.
Abstract
In this research a set of 12 simple sequence repeat (SSR) markers was used to evaluate the genetic diversity background of 11 pomegranate (Punica granatum L.) cultivars were collected from the agricultural directorate nursery in Duhok province. These cultivars represent the main cultivars which cultivated in Kurdistan region, particularly around Duhok province. Among the developed primers, five revealed monomorphic alleles across all pomegranate populations, whereas seven loci amplified polymorphic banding patterns. A total of 25 alleles were detected with average of 2.08 alleles per locus. The mean values of expected (He) heterozygosity were 0.3315. The content average of polymorphic information was 0.2572. Genetic distance among the 11 varieties ranged from 0.0606 to 0.2683. Results of overall data and cluster analysis suggested that this set of primers can be very useful for studding genetic diversity and pedigree analysis of this important economical important fruit species.
Keywords
Pomegranate (Punica granatum L.), Microsatellite, Genetic Diversity
References
[01] Alamutia, M. K.; Zeinalabedini, M.; Derazmahalleh, M. M.; RoodbarShojaie, T.; Irandoost, H. P.; Zahravi, M.; Vazifehshenas, M.; Ebrahimi, M. A.; Nekouei, S. M.; Salekdeh, G. H. and Mardi, M. (2012). Extensive genetic diversity in Iranian pomegranate (Punica granatum L.) germplasm revealed by microsatellite markers. Scientia Horticulturae 146, 104–114.
[02] Anonymous, (2005). Report of the USDA national clonal germplasm respiratory (NCGR),Davis,CA. http://www.ars.usda.gov/sp2UserFiles/Place/53481500/reports/ 2005AnnualReport_Davis.pdf 2005.
[03] Botstein, D, White, RL, Skolnick, M and Davis, RW (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Amer. J. of Human Genetics 32, 314-331.
[04] Doyle, J. J. and Doyle J. L. (1990). A rapid total DNA preparation procedure for fresh plant tissue. Focus 12:13-15.
[05] Doyle, J. J. (1991). DNA protocols for plants. pp. 283-293 in: G. Hewitt, A. W. B.Johnson, and J. P. W. Young (eds.), Molecular Techniques in Taxonomy. NATO ASI Series H, Cell Biology Vol. 57.
[06] Durgac, C.; Özgen, M.; Simsek, Ö.; Kacar, A.Y.; Kıyga, Y.; Celebi, S.; Gündüz, K. and Serce, S. (2008). Molecular and pomological diversity among pomegranate (Punica granatum L.) cultivars in Eastern Mediterranean region of Turkey. Afr. J. Biotechnol. 7, 1294–1301.
[07] Ebrahimi, S.; Ebrahim, B. S. and Sharifnabi, B. (2010). Microsatellite isolation and characterization in pomegranate (Punica granatum L.). Iranian J. of Biotech. 8(3); 156-163.
[08] Engels, J.M.M. and Visser, L. (2003). A guide to Effective Management of Germplasm Collections. P. 174
[09] Gupta, P.K.; Balyan, H.S.; Sharma, P.C. and Ramesh, B. (1996). Microsatellites in plants: a new class of molecular markers. Curr. Sci. 70:45–54.
[10] Hasnaoui, N.; Mars, M.; Chibani, J. and Trifi, M. (2010a). Molecular Polymorphisms in Tunisian Pomegranate (Punica granatum L.) as Revealed by RAPD Fingerprints. Diversity 2: 107-114.
[11] Hasnaoui, N.; Buonamici, A.; Sebastiani, F.; Mars, M.; Trifi, M. and Vendramin, G. G. (2010b). Development and characterization of SSR markers for pomegranate (Punica granatum L.) using an enriched library. Conservation Genet Resources. 2(1):283-285.
[12] Hasnaoui, N.; Buonamici, A.; Sebastiani, F.; Mars, M.; Zhang, D. and Vendramin G. G. (2011). Molecular genetic diversity of Punica granatum L. (pomegranate) as revealed by microsatellite DNA markers (SSR). Elsevier B.V. Gene 493(1): 105-112.
[13] Holland, D.; Hatib, K. and Bar-Ya'akov, I. (2009). Pomegranate: botany, horticulture, breeding. Hortic. Rev. 35, 127–191.
[14] Huang, Y. and Shi, S. (2002). Phylogenetics in the Lythraceae sensu lato: A preliminary analysis based on plastic rbcL and psaA-ycf3 spacer, and ITS of nrDNA sequences. Inte. J. of Plant Sci. 163:215–225.
[15] IPGRI, (2002). Neglected and Underutilized Plant Species: Strategic Action Plan of the International Plant Genetic Resources Institute. International Plant Genetic Resources Institute, Rome, Italy. P. 27
[16] Jbir, R.; Hasnaoui, N.; Mars, M.; Marrakchi, M. and Trifi, M. (2008). Characterization of Tunisian pomegranate (Punica granatum L.) cultivars using amplified fragment length polymorphism analysis. Sci. Hort. 115, 231–237.
[17] Mars, M. (2001). Ressources génétiques du grenadier (Punica granatum L.) en Tunisie: prospection, conservation et analyse de la diversité. Thèse d'état, Faculté des Sciences de Tunis, Tunisia.
[18] Marshal, T.C.; Slate, J.; Kruuk, LEB.; Pemberton, J.M. (1998).Statistical confidence for likelihood-based paternity inference in natural populations. Mol. Ecol. 7, 639–655.
[19] Nei, M. (1987). Molecular Evolutionary Genetics. Columbia University Press: New York.
[20] Nei, M. and Li, W. H. (1979). Mathematical moder for studding genetic variation in terms of restriction endonuclease. Proc. Nat. Acad. Sci. U.S.A. 74:5269-5273
[21] Pirseyedi, S.M.; Valizadehghan, S.; Mardi, M.; Ghaffari, M.R.; Mahmoodi, P.; Zahravi, M.; Zeinalabedini, M. and Nekoui, S.M.K. (2010). Isolation and characterization of novel microsatellite markers in pomegranate (Punica granatum L.). Int. J. Mol. Sci. 11 (5), 2010–2016.
[22] Priolli, R.H.G.; Mendes-Junior, C.T.; Arantes, N.E. and Contel, E.P.B. (2002). Characterization of Brazilian soybean cultivars using microsatellite markers. Genet Mol Biol. 25: 185-193.
[23] Ranade, S.A.; Rana, T.S. and Narzary, D. (2009). SPAR profiles and genetic diversity amongst pomegranate (Punica granatum L.) genoypes. Physiol. Mol. Biol. Plants, 15, 61–70.
[24] Rice, W.R. (1989) Analyzing tables of statistical tests. Evolution: Int. J. Org. Evol. 43, 223–225.
[25] Rohlf, F. J. (1993). NT SYS-PC. Numerical Taxonomy and Multivariate Analysis System. Version 1.8 Exter Software, Setauket, New York, U.S.A.
[26] Rousset, F. (2008). Genepop'007: a complete reimplementation of the Genepop software for Windows and Linux. Mol. Ecol. Resources 8: 103-106.
[27] Sarkhosh, A.; Zamani, Z.; Fatahi, R. and Ebadi, A. (2006). RAPD markers reveal polymorphism among some Iranian pomegranate (Punica granatum L.) genotypes. Sci. Hortic. 111, 24–29.
[28] Seeram, N. P.; Schulman, R. N. and Heber, D. (2006). Pomegranate: ancient roots to modern medicine. CRC Press &Taylor and Francis Group, Boca Raton, FL, USA. pp. 5-7
[29] Sergio, C.; Marco, C.; Gaetano, D.; Alessandra, G. and Stefano La, M. (2010). New microsatellite loci for pomegranate, punica granatum (lythraceae). AJB Primer Notes and Protocols in the Plant Sciences, American Journal of Botany, 58–60.
[30] Sinjare D.Y. (2013). Microsatellite application and genetic characterization of Pomegranate (Punica granatum L.) in Duhok Province/ Kurdistan Region- Iraq. A thesis of M.Sc.
[31] Soriano, J.M.; Zuriaga, E.; Rubio, P.; Llácer, G.; Infante, R. and Badenes, M.L. (2010). Development and characterization of microsatellite markers in pomegranate (Punica granatum L.). Molecular Breeding, 27, 119–128.
[32] Still, D.W. (2006). Pomegranate: a botanical perspective. In: Seeram, N.P., Schulman, R.N., Heber, D. (Eds.), Pomegranates: Ancient roots to modern medicine. CRC Press, Taylor and Francis, Florida, pp. 199- 209.
[33] Sturgeon, S.R. and Ronnenberg, A.G. (2010). Pomegranate and breast cancer: possible mechanism of prevention. Nutr. Rev. 68, 122–128.
[34] Talebi-Baddaf, M.; Sharifi-Neia, B. and Bahar, M. (2003). Analysis of genetic diversity in pomegranate cultivars of Iran, using Random Amplified Polymorphic DNA (RAPD) markers. Proceedings of the Third National Congress of Biotechnology, Iran, pp. 343–345.
[35] Tezcan, F.; Gültekin-Özgüven, M.; Diken, T.; Özçelik, B. and Erim F.B. (2009). Antioxidant activity and total phenolic, organic acid and sugar content in commercial pomegranate juices. Food Chem. 115, 873–877.
[36] Townsend C.C. and Guest E. (1964). Flowra of Iraq. Ministry of agriculture.
[37] Weir, B.S. (1990). Genetic data analysis. Methods for discrete genetic data. Sinauer Associates, Suderland. P. 445.
[38] Zamani, Z.; Sarkhosh, A.; Fatahi, R. and Ebadi, A. (2007). Genetic relationships among pomegranate genotypes studied by fruit characteristics and RAPD markers. J. Hortic. Sci. Biotechnol, 82; 11-18.
[39] Zhang, D.; Mischke, S.; Johnson, E.S.; Philips-Mora, W. and Meinhardt, L., (2009). Molecular characterization of an international cacao collection using microsatellite markers.Tree Genet. Genomes, 5, 1–10.
600 ATLANTIC AVE, BOSTON,
MA 02210, USA
+001-6179630233
AIS is an academia-oriented and non-commercial institute aiming at providing users with a way to quickly and easily get the academic and scientific information.
Copyright © 2014 - American Institute of Science except certain content provided by third parties.