International Journal of Chemical and Biomolecular Science
Articles Information
International Journal of Chemical and Biomolecular Science, Vol.1, No.3, Oct. 2015, Pub. Date: Sep. 2, 2015
Spectrophotometric Quantification of Vardenafil in Bulk and Tablets
Pages: 185-192 Views: 2507 Downloads: 1368
Authors
[01] Atkuru Veera Venkata Naga Krishna Sunil Kumar, Department of Chemistry, NRI Institute of Technology, Pothavarappadu, Andhra Pradesh, India.
[02] Chandra Bala Sekaran, Department of Pharmaceutical Biotechnology and Pharmaceutical Analysis, Medarametla Anjamma Mastan Rao College of Pharmacy, Narasaraopet, Andhra Pradesh, India.
[03] Tamanampudi Varahala Reddy, Department of Chemistry, Mallareddy College of Engineering, Secundrabad, Andhra Pradesh, India.
Abstract
The purpose of the present investigation is to develop and validate three spectrophotometric methods (1, 2 & 3) for the determination of vardenafil (VDL). Method 1 is based on the oxidation of VDL with KMnO4 in 0.6 M NaOH at room temperature and measurement of the green colored manganate ions at 630 nm. Method 2 involves the formation of yellow colored chloroform extractable ion-pair complex of VDL with bromocresol green under acidic condition. The colored ion-pair complex is quantitated spectrophotometrically at 420 nm. Method 3 is based on the inner molecular complex formation of the VDL with acetaldehyde and sodium nitroprusside in alkaline medium. The colored inner molecular complex is measured at 560 nm. Under the optimized experimental conditions, the absorbance vs concentration plot was linear over the range of 10-100 µg/ml, 5-60 µg/ml and 2-20 µg/ml with molar absorptivities of 4.299 x 103, 3.755 x 104 and 4.079 x 104 L/mole/cm for methods 1, 2 and 3, respectively. The limits of detection and quantification values were calculated to be 0.335 & 1.015 µg/ml (method 1), 0.055 & 0.166 µg/ml (method 2) and 0.013 & 0.039 µg/ml (method 3). The relative standard deviations obtained in the intra- and inter-day analyses were in the range of 0.082%-0.890% (method 1), 0.114%-1.515% (method 2) and 0.372%-0.942% (method 3). The methods are successfully applied to the quantification of VDL tablets with percentage recovery in the range of 99.30-99.88% (method 1), 99.60-99.88% (method 2) and 99.80-99.90% (method 3). Common excipients present in tablet formulations did not interfere with the assay. The results demonstrate that the proposed methods are accurate, reproducible and can be useful for routine analysis of VDL in tablets.
Keywords
Vardenafil, Ion-Pair Complex, Oxidoreduction, Inner Molecular Complex, Spectrophotometric Analysis
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