International Journal of Chemical and Biomolecular Science
Articles Information
International Journal of Chemical and Biomolecular Science, Vol.1, No.4, Dec. 2015, Pub. Date: Nov. 12, 2015
Phylogenetic Grouping of Uropathogenic Escherichia coli Using Different Molecular Typing Methods in Kurdistan Region-Iraq
Pages: 284-291 Views: 1680 Downloads: 4614
Authors
[01] Narmin S. Merza, Scientific Research Center, Duhok University, Duhok, Iraq.
[02] Jaladet M. S. Jubrael, Scientific Research Center, Duhok University, Duhok, Iraq.
Abstract
A total of 364 clinical isolates of Escherichia coli were collected and characterized from different hospitals in Kurdistan region. Two hundred ninety six of these were isolated from female accounting (81%) whereas 68(19%) of them were recovered from male. One hundred fifty isolates of UPEC (Fifty from each province) were randomly selected and confirmed as E. coli isolates at molecular level by PCR using uidA primer as a species-specific primer producing 670bp DNA fragment for uidA gene. They were assigned into one of the major phylogenetic groups (A, B1, B2 and D). This is based on the presence or absence of genetic markers including: two genes (chuA and yjaA) and a DNA fragment (TSPE4.C2) as proposed by Clermont et al., 2000. It has been found that 85 (56.7%) of these isolates belonged to phylogenetic group B2, 31 (20.7%) belonged to group D, 27 isolates were phylo-group (A) a counting (18%), and only 7 isolates (4.7%) belonged to group B1. Further classification has been done in order to increase the discrimination power of E. coli population analyses by classification these phylogroups into seven subgroups including, A0, A1, B1, B22, B23, D1and D2. Phylogenetic group B2 showed two types of gene patterns. Seven isolates belonged to B22 and 77 isolates designated as B23. In addition to unusual strain (-++) which assigned to phylogroup B2. Phylogenetic group D revealed two gene patterns; it has been found that 11 of them were belonging to subgroup D1 and 20 isolates belonged to sub group D2. In the third phylogroup A, only 2 isolates belonged to subgroup A0 and 25 isolates belonged to subgroup A1. Out of 7 isolates which belonged to Phylogroup B1, 2 isolates had unique pattern and assigned as B1a. From these results it have been found that B23 (+++) was the most predominant among all other subgroups accounting (77/150) 51.3%, while subgroup A0 (---) was the least prevalent among all others accounting (2/150) 1.3%.
Keywords
Phylogenetic Grouping, Triplex PCR, Escherichia coli
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