International Journal of Chemical and Biomolecular Science
Articles Information
International Journal of Chemical and Biomolecular Science, Vol.2, No.4, Aug. 2016, Pub. Date: Jul. 21, 2016
Best Method for the Extraction of Egg Carotenoid Pigments Golden Egg Snails (Pomacea canaliculata Lamarck)
Pages: 69-72 Views: 2372 Downloads: 1343
Authors
[01] Nurjanah Taufik Hidayat, Department Aquatic Product Technology, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor West Java, Indonesia.
[02] Batari Perbawani, Department Aquatic Product Technology, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor West Java, Indonesia.
Abstract
Snails (Pomacea canaliculata Lamarck) is one type of freshwater snails which comes from the Americas. Snails have become a serious threat in agriculture due to high reproductive power and capability of rapid deployment area. This research result of chemical composition showed that golden snail egg contain to high moisture and fat. Golden snail eggs have a high total carotenoids is 313.48 ± 19.73 ppm. This research aims to optimize the extraction process of carotenoids with different types of solvents. Golden snail eggs, chopped and mashed in a blender then dried by the method of freeze dry for 58 hours. After getting the golden snail eggs, egg powder snails do multilevel extraction method using n-hexane, acetone and methanol in the ratio 1: 10 (w / v) and extracted with maceration for 24 hours. Furthermore, the single extraction with solvents methanol 1: 10 (w / v), ethanol 1: 10 (w/v), acetone: methanol with a ratio of 3: 7 and acetone: distilled water with a ratio of 3: 15 (w/v) method maceration for 48 hours. Results extraction then evaporated and the result filtrate diluted with methanol measured using a spectrophotometer to see the absorption wavelength. The optimum solvent extraction golden snail eggs is a polar solvent to extract the highest acetone: distilled water with a ratio of 3: 15 namely 1084.6 mg.
Keywords
Carotene, Egg Snails, Chemical Composition, Extraction
References
[01] Azka A, Nurjanah, Jacoeb AM. 2015. Profil asam lemak, asam amino, total karotenoid, dan α- tokoferol telur ikan terbang. Jurnal Pengolahan Hasil Perikanan Indonesia. 18 (3): 250-261.
[02] Belitz HD, Grosch W, Schieberle P. 2009. Food Chemistry. Ed rev ke-4. Verlag: Springer.
[03] Briton G, S. L Jansen dan H. Pfander. Carotenoids. 1995. Vol IB. Spectroscopy. Academic Press Inc. Basel 386.
[04] Budiyono S. 2006. Teknik mengendalikan keong mas pada tanaman padi. Jurnal Ilmu-Ilmu Pertanian 2(2): 128-133.
[05] Cappeli B dan Cheswky G. 2007. Natural Astaxhantin. USA: Cyanotech Coorporation.
[06] Darwis, D. 2000. Teknik Dasar Laboratorium dalam Penelitian Senyawa Bahan Alam Hayati. Workshop Pengembangan Sumber Daya Manusia dalam Bidang Kimia Organik Bahan Alam Hayati. FMIPA Universitas Andalas. Padang.
[07] Desiana. 2000. Ekstraksi pigmen karotenoid dari limbah kulit udang windu (Penaeus monodon Fabricus) dengan bantuan enzim papain [skripsi]. Bogor: Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor.
[08] Dreon MS, Ituarte S, Ceolin M, Heras H. 2008. Global shape and pH stability of ovorubin, an oligomeric protein from the eggs of Pomacea canaliculata. FEBS Journal 275 (18): 4522-4530.
[09] Henrikson R. 2009. Earth Food Spirulina How this Remarkable Blue-Green Algae can Transform Your Health and Our Planet. USA: Ronore Enterprises. Inc.
[10] Heras H, Pollero RJ. 2002. Lipoprotein from plasma and perivelline fluid of the apple snail Pomacea canaliculata. Biocell 26(1): 111-118.
[11] Nurjanah N, Nurhayati T, Hidayat T, Agustina MA. 2016. Mineral content of Macro-Micro And Total Carotenoids Eggs Golden Apple Snail (Pomacea canaliculata) From Pond Culture FPIK IPB. International Food Research Journal. Inpress.
[12] Mortensen A. 2006. Carotenoids and Other Pigments as Natural Colorants. Pure Appl. Chem.. 78 (8): 1477–1491.
[13] Slamet Sudamadji, Barnbang Haryono, Suhardi. 1996. Analisa Bahan Makanan dan Pertanian. Yogyakarta: Liberty.
[14] Wu YL, Yang CC. 2008. Method for obtaining natural astaxanthin from eggs and gonads of snails, US patent and trade mark office Patent US8030523.
[15] Yulianingsih R, Tamzil. 2007. Analisis proksimat rumput laut produksi dari beberapa lokasi di Indonesia Timur. Buletin Teknologi Litkayasa Akuakultur 1(6): 51-55.
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