International Journal of Chemical and Biomolecular Science
Articles Information
International Journal of Chemical and Biomolecular Science, Vol.4, No.3, Sep. 2018, Pub. Date: Sep. 4, 2018
Assessment of Hydrocarbon-Utilizing Microorganisms from Spent Engine Oil-Polluted Soils from Mechanic Workshops in Awka Metropolis
Pages: 33-40 Views: 117 Downloads: 114
Authors
[01] Okafor Ugochukwu Chukwuma, Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Nigeria.
[02] Nwose Onyeka Divine, Department of Applied Microbiology and Brewing, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Nigeria.
Abstract
The hydrocarbon utilizing potential of microorganisms from spent engine oil-polluted soils was evaluated. Samples were collected from spent engine oil-polluted soils from three mechanic workshops at Ifite, Arroma and Kwata in Awka metropolis, Anambra state Nigeria. The total heterotrophic Indigenous bacterial count was evaluated and oil polluted soil sample obtained from mechanic workshop in Arroma had the highest count with a colony count of 3.5×106cfu/g; soils obtained from Ifite and Kwata mechanic workshop had colony counts of 3.0×106cfu/g and 2.5×106cfu/g respectively. The Total Fungal count was also evaluated and soil samples from Kwata mechanic workshop showed the highest count with 3.9×105cfu/g; colony counts of soils obtained from Ifite and Arroma mechanic workshop were 2.0×105cfu/g and 2.5×105cfu/g respectively. The Hydrocarbonoclastic microbial count showed that soil samples from mechanic workshop in Arroma had the highest load of hydrocarbonoclastic bacteria with a colony count of 4.1×105cfu/g while soil samples from mechanic workshop in Kwata had the highest load of hydrocarbonoclastic fungi with a colony count of 3.3×105cfu/g. The morphological and biochemical characteristics of the Hydrocarbonoclastic organisms were examined and the following genera were identified and they include: Pseudomonas (25%), Bacillus (25%), Acinetobacter (12.5%), Staphylococcus (12.5%), Micrococcus (12.5%),) and Enterobacter (12.5%),); Aspergillus (33.3%), Rhizopus (16.6%), Fusarium (33.3%) and Penicillium (16.6%); Candida (66.7%) and Trichosporon (33.3%). This study showed that majority of the Indigenous organisms has the ability to utilize hydrocarbon as their sole carbon source hence are the major bioremediating agents in the sampling areas.
Keywords
Spent Engine Oil, Biostimulation, Hydrocarbonoclastic Organisms, Soil, Awka
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