International Journal of Chemical and Biomolecular Science
Articles Information
International Journal of Chemical and Biomolecular Science, Vol.4, No.4, Dec. 2018, Pub. Date: Dec. 21, 2018
Effect of Storage on the Bioactivity of Drepanoalpha® (An Anti-Sickle Cell Disease Polyherbal Formula) and Comparative Biochemical Profile of Different Batches
Pages: 60-68 Views: 393 Downloads: 184
Authors
[01] Koto-te-Nyiwa Ngbolua, Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo; Department of Biomedical Sciences, University of Gbadolite, Gbadolite, Democratic Republic of the Congo.
[02] Benjamin Zoawe Gbolo, Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo.
[03] Joseph Dipa Tshidibi, Department of Biology, University of Kinshasa, Kinshasa, Democratic Republic of the Congo.
[04] Damien Sha Tshibey Tshibangu, Department of Chemistry, University of Kinshasa, Kinshasa, Democratic Republic of the Congo.
[05] Patrick Bondo Memvanga, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo.
[06] Pius Tshimankinda Mpiana, Department of Chemistry, University of Kinshasa, Kinshasa, Democratic Republic of the Congo.
Abstract
Drepanoalpha® is a poly-herbal formula (PHF) used in Democratic Republic of the Congo for the management of Sickle Cell Disease (SCD), a genetic disease due to the presence of hemoglobin S in the blood which could in hypoxia conduct to the formation of tactoids, the leading cause of erythrocyte sickling that plays a key role in the pathophysiology of SCD like vaso-occlusion (due to the loss of membrane elasticity) and hemolytic anemia. The aim of the present study was to evaluate the storage time effect on the bioactivity and the secondary metabolites (anthocyanis and flavonoids) and minerals (Fe, Mn, Zn and Cu) profiles of this PHF. Results revealed that flavonoids content of different batches is of the same order of magnitude: the percentage of ratio flavonoids/total polyphenols was 5.3% (batch A); 5.0% (batch B); 4.7% (batch C). However, the anthocyanins content of PHF varies according to the storage time. The percentage of ratio anthocyanis/total polyphenols was 3, 2% for batch A; 4, 7% for batch B; 8, 3% for batch C. Results revealed no significant difference between the average values of total ash (11.34 ± 0.35 84 g/100 g DM for A; 11.5 ± 0.12 84 g/100 g DM for B and 10 ± 0.84 84 g/100 g DM for C) of three batches (threshold: 5%). The different batches of Drepanoalpha® contain Fe (0.535± 0.107 mg/100 g for A; 0.517 ± 0.103 mg/100 g for B; 0.54 ± 0.108 mg/100 g for C), Mn (4.366 ± 0.786 mg/100 g for A; 4.7 ± 0.799 mg/100 g for B; 4.318 ± 0.734 mg/100 g for C), and Zn (2.293 ± 0.321 mg/100 g for A; 2.389 ± 0.334 mg/100 g for B; 2.338 ± 0.327 mg/100 g for C) and are free of Cu. The micronutrient values of batches A, B and C are not significantly different (threshold: 5%) from each other. At 250 μg /mL, there is no significant difference (threshold: 5%) in anti-oxidative activity between the different batches. All samples displayed strong anti-sickling activity indicating thus at room temperature, the storage time (one or two years) has no effect on the bioactivity, flavonoids content and mineral profile of the PHF. It is desirable that Drepanoalpha® could be formulated as syrup or capsule for a double blind, placebo-controlled and randomized cross-over clinical trials in SCD patients.
Keywords
Sickle Cell Disease, Traditional Medicine, Medicinal Foods, Anti-sickling Activity, Polyphenols
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