International Journal of Life Science and Engineering
Articles Information
International Journal of Life Science and Engineering, Vol.1, No.3, Jul. 2015, Pub. Date: Jun. 24, 2015
Antitrypanosomal Activity of Leaf Extracts of Acalypha Wilkesiana
Pages: 114-119 Views: 2986 Downloads: 2527
Authors
[01] Sani B. Saleh, Department of Vector and Parasitology, Nigerian Institute for Trypanosomiasis Research (NITR), Kaduna, Nigeria.
[02] Toma Ibrahim, Department of Vector and Parasitology, Nigerian Institute for Trypanosomiasis Research (NITR), Kaduna, Nigeria.
[03] Abdulkadir R. Salisu, Department of Environmental Science, School of Environmental Studies, Kaduna Polytechnic, Kaduna, Nigeria.
[04] Stephen S. Shaida, Department of Vector and Parasitology, Nigerian Institute for Trypanosomiasis Research (NITR), Kaduna, Nigeria.
[05] Haruna Jibril, Department of Vector and Parasitology, Nigerian Institute for Trypanosomiasis Research (NITR), Kaduna, Nigeria.
[06] Isadu Habu Tela, Department of Vector and Parasitology, Nigerian Institute for Trypanosomiasis Research (NITR), Kaduna, Nigeria.
[07] Saminu S. Abdullahi, Department of Vector and Parasitology, Nigerian Institute for Trypanosomiasis Research (NITR), Kaduna, Nigeria.
[08] Elizabeth A. Adekemi, Department of Vector and Parasitology, Nigerian Institute for Trypanosomiasis Research (NITR), Kaduna, Nigeria.
[09] Hadiza M. Kabir, Department of Vector and Parasitology, Nigerian Institute for Trypanosomiasis Research (NITR), Kaduna, Nigeria.
[10] Idris Bala, Department of Microbiology, Bayero University, Kano, Nigeria.
[11] Abdurrahim Suleiman, Department of Vector and Parasitology, Nigerian Institute for Trypanosomiasis Research (NITR), Kaduna, Nigeria.
Abstract
Acalypha wilkesiana (Red Hot Cat’s Tail) has reportedly being used to treat a number of fungal (Eukaryotic) infections and so, might also have trypanocidal (parasitic- eukaryotic) properties. In this study, Ethanolic and water extracts of Red Hot Cat’s Tail plant were graded into concentrations of (30, 15, 7.5 and 3.73mg/ml) and tested against actively motile Trypanosoma brucei brucei. The extracts were then screened for alkaloids, tannins, saponins, cardiac glycosides, amino acid, reducing sugar, steroids, triterpanoids, and anthraquinones. At 30mg/ml, complete cessation of motility of the parasite occurred within 25min (for aqueous extract) and 30min (for ethanolic extract). At 15mg/ml, 7.5mg /ml, 3.72mg/ml of both the extracts, complete cessation of motility of the parasites within 40mins, 45mins and 50mins was observed respectively. High activity of the leaf extracts against T. brucei brucei might not be unconnected with the presence of efficient bioactive compounds in it. Therefore, this plant can locally be used against T. brucei brucei infestation.
Keywords
Acalypha Wilkesiana, Trypanosoma Brucei Brucei, Extract, Concentration, Phytochemicals
References
[01] Oyelami, O.A. Onayemi, O. Oladimeji, A. Onawunmi, O. (2003). Clinical Evaluation of Acalypha ointment in the treatment of superficial fungal skin diseases. Phytotherapy Research. Wiley Interscience, 17: 555-557.
[02] Akinyemi K., Oladapo O., Okawara C. and Fasure (2006), screening of crude extract of six medicinal plants used in southwest Nigerian orthodox Medicine for Anti-Methicillin Resistant Staphylococcus aureus Activity. BMC Complementary and Alternative Medicine 1-7.
[03] Nagararajan S.M., Suresh T. Rajasekaran S., Kannan T.M.S., and Kulothungan S. (2005), Invitro Micropropagation of Boerhaavia diffusa L. Geobios-Jodhpur 32:169-172.
[04] Deborggraeve S., Koffi M. and Jamonneau V. (2008). Molecular analysis of archived blood slides reveals an atypical human Trypanosoma infection. Diagn. Microbiol. Infect. Dis. 61 (4): 428–33.
[05] Masocha W., Kristensson K. (2012). "Passage of parasites across the blood-brain barrier" Virulence 3 (2): 202–12.
[06] Barrett MP, Burchmore RJ, Stich A, Lazzari JO., Frasch AC., Cazzulo JJ and Krishna S. (2003). "The trypanosomiases". Lancet 362 (9394): 1469–80.
[07] Stephens N.A., Kieft R., Macleod A., Hajduk S.L. (2012). "Trypanosome resistance to human innate immunity: targeting Achilles' heel". Trends in Parasitology. 28 (12): 539–45.
[08] Herert W.J. and Lumsden W.H. (1976) Experimental Parasitology 40(3): 427-431.
[09] Atawodi, S.E Bulus, T. Ibrahim, S. Ameh, D.A., Nok, A.J., Muhammad M. and Galadima, M. (2003). In vitro Trypanosomal Effect of Methanolic Extract of Some Nigerian Savannah Plants, Africa Journal of Biotechnology 2(9): 317-321.
[10] Wang, W., Vignani, R., Scaly, M. and Christy, M. (2006). A Universal and Rapid Protocol for Protein Extraction from Recalcitrant Plant TX for Protemic Analysis, Electrophoresy, 27: 2782-2786.
[11] Poongothai, A., Sreena, K.P., Sreejith, K., Uthiralingam M. and Annapoorani, S. (2011). Preliminary phytochemicals screening of ficus racemosa linn back, International Journal of Pharmaceutical and Biological Sciences vol. 2 issue 2, ISSN 0975-6299.
[12] Akintobi, O. A., Onoh C. C., Ogele JO., Idowu AA., Ojo OV. and Okonko IO. (2013) Antimicrobial activity of Zingiber officinale (Ginger) extract against some selected pathogenic bacteria, Nature of science, 11(1).
[13] Gull I., Maryam S., Halima S., Shabaz M., Aslam, Z., Qadir S. and Amin M. A. (2012). Inhibitory Effect of Allium sativum and Zingiber officinale extracts on clinically important drug resistantpathogenic bacteria, Annals of Clinical Microbiology and Antimicrobials 11:18.
[14] Augusti K.T. and Cherian S. (2008). Insulin sparing action of leucopelargonidin derivative isolated from Ficus bengalosis Linn. Indian Journal of Experimental Biology 33:608-611.
[15] Rupasinghe, H. P. V., Jackson, C. J. C., Poysa, V., Di Berardo, C., Bewley, J. D. and Jenkinson, J. (2003). Soyasapogenol A and B distribution distribution in Soybean (Glycin max I., Merr.) in Relation to Seed Physiology, Genetic Variability and Growing Location Journal of Agricultural and Food Chemistry 51(20), 5888-5894.
[16] Mandal S., Bibhabasu H., Rhitajit S., Santanu B. and Nripendranath M. (2009), Hemidesmus Indicus, an Age Old Plant: Study of its Invitro Antioxidant and Free Radical Scavenging Potentials. Division of Molecular Medicine, Bose Institute, India.
[17] Shaikh R. J, Paurani M P., Mali P R., Karigar A. and Yeole P. G. (2010). Sensitive and Selective Analytical Method for the Quantification of Glipizide in Human Plasma: International Research Journal of Pharmacy 1(1): 378-383.
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