International Journal of Life Science and Engineering
Articles Information
International Journal of Life Science and Engineering, Vol.1, No.1, Mar. 2015, Pub. Date: Feb. 15, 2015
Rapid Diagnosis of Acute Respiratory Infections by Multiplex Endpoint PCR Technology
Pages: 1-6 Views: 2833 Downloads: 1382
Authors
[01] Aurelian Udristioiu, Emergency County Hospital Targu Jiu & UCB University, Clinical Laboratory, City Targu Jiu, Romania.
[02] Manole Cojocaru, TituMaiorescu University, Faculty of Medicine, Physiology Department, City Bucharest, Romania.
[03] Dana Alexandra Maria Panait, TituMaiorescu University, Faculty of Medicine, Physiology Department, City Bucharest, Romania.
[04] Nica Badea Delia, Constantin Brancusi University, Faculty of Medical Science and Behaviors, City Targu Jiu, Romania.
Abstract
Introduction: PCR offer a number of potential advantages, results are available in a matter of hours rather than days, the extreme sensibility facilitates detection of even minutes the amounts of pathogen DNA in clinical samples and the test is not significantly affected by prior administration of antibiotics. Aim: The aim of this work was to rapidly identify the antibiotic resistance the monitoring of pathogen grow that the patients admitted in Hospitalization Intensive Care Unit of Emergency County Hospital Targu Jiu starting in December/2013. Method: The Analyzer Unyvero™ Pneumonia Application was used in detection of pneumonia associated pathogens and their antibiotic resistance genes using the Unyvero™ System following PCR, pathogen species with sequencing of the amplified microbial DNA. Results: The main pathogens of community acquired pneumonia are Streptococcus pneumonia (5 cases), and Klebsiella pneumonia (4cases), other important agents are “atypical”, such as Haemophilus Influenzae, Chlamidophila pneumonie and Moraxela cataralis. A case with Acinetobacter baumani and Proteus Sp.are also widely resistance to mefA gene / ermB gene as all cases of analyzed. The more frequency of gene resistant (9 cases) are ermA gene / ermC / ermB for Staphilococcus aureus and the gene tem+shv / gene / ctx-M with the Chromosomal mutation (4 cases), as gyrA83_87 Ecoli / Pseu for Klebsiella pneumonia agents. Also most resistance antibiotics was Makrolides (9 cases and Lincosamides (4 cases) and this cases have chromosomial integrates. The most resistance microbe, Pseudomonas aeruginosa (1 case), has registered multi drugs resistance [MDR]*.Conclusions: The Unyvero™ results were available 2 days before the primary microbiology report and 3 days before the final confirmation results, obtained by microbiology culture. The Unyvero Analyzer only provides rapid data to support the therapeutic decision of currant medic.
Keywords
Antibiotic Resistance, PCR, Gene, DNA, Resistance Markers, Microbiology Report
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