International Journal of Plant Science and Ecology
Articles Information
International Journal of Plant Science and Ecology, Vol.1, No.6, Dec. 2015, Pub. Date: Jan. 6, 2016
Comparison of Antimicrobial Properties of Silybum marianum (L) Collected from Ten Different Localities of Khyber Pakhtunkhwa Pakistan and Diversity Analysis Through RAPDs Pattern
Pages: 241-245 Views: 1263 Downloads: 1201
Authors
[01] Nisar Ahmad, Department of Botany, Kohat University of Science and Technology, Kohat, Pakistan.
[02] Razia Perveen, Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Pakistan.
[03] Muhammad Jamil, Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Pakistan.
[04] Rehan Naeem, Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Pakistan.
[05] Muhammad Ilyas, Department of Botany, Kohat University of Science and Technology, Kohat, Pakistan.
Abstract
The Silybum marianum was collected from ten different localities of Khyber Pakhtunkhwa Pakistan to investigate the effects of diverse environmental conditions on the antimicrobial profile and genetic diversity of the plant and to look into possible natural therapy. In antibacterial assay it was explored that the methanolic and n-hexane fractions of all the localities exhibited insignificant results, while the chloroform fraction showed profound results with inhibition percentage 58.8, 52.9 and 47.1 from Charsada, Karak and Bannu regions respectively against E. coli whereas the fractions from Bannu, Kohat and Lakimarwat showed 57.9%, 52.6% and 47.4% inhibition against Vibrio cholerae. The average percentage inhibition of the samples from Peshawar, North Waziristan, Abbottabad, Hangu and Lakimarwat was 45 percent against Shigella species. Moderate percentage of inhibition was noticed against Staphylococcus aureus by the fractions of Lakimarwat (42.1%), each 36.8 % for Waziristan and Abbottabad. No antifungal activity was showed by any fraction used. Using RAPP markers OPE7, the genetic diversity analysis results revealed two monomorphic bands of 600bp and 450bp for all the samples of Silybum marianum collected from different locations. All the bands observed have no differentiateable association with antibacterial and antifungal profiles. The bioactive fractions of the plants can be used against various microbial infections and can be further used for the isolation of potentials natural product for drug development.
Keywords
Silybum marianum, Antimicrobial, Antifungal, Drug Sighting, Genetic Diversity, RAPDs
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