International Journal of Plant Science and Ecology
Articles Information
International Journal of Plant Science and Ecology, Vol.5, No.2, Jun. 2019, Pub. Date: Jul. 12, 2019
Investigation of the Antioxidant Activity of Aqueous and Ethanol Leaf Extracts of Ginkgo biloba from South-East Nigeria
Pages: 31-36 Views: 74 Downloads: 38
Authors
[01] Nwosu Onyeka Kingsley, Department of Applied Biochemistry, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria; National Biosafety Management Agency, Abuja, Nigeria.
[02] Ubaoji Kingsley Ikechukwu, Department of Applied Biochemistry, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.
[03] Okaka Antoinette Nwamaka Chinwe, Department of Applied Biochemistry, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.
[04] Okeke David Okechukwu, Department of Applied Biochemistry, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.
Abstract
The leaf extracts of Ginkgo biloba (GB) have been used extensively over the years in Asia and South America for health beneficial effects to human, however the rarely grown GB in Nigeria is yet to be grossly tapped. In this study, we examined the antioxidant potentials of GB leaf extracts (aqueous and ethanol) from Nigeria. Non-enzymatic and enzymatic methods were used to determine the antioxidant activities while Association of Official Analytical Chemist (AOAC) method was used to determine the β-carotene concentration in both extracts and vitamin C and E composition in the whole dried leaves. The antioxidant analysis showed that ethanol extract exhibited more antioxidant properties than the aqueous extract. However, both extracts were able to show very considerable 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide radical scavenging activity, and antioxidant activity observed from β-carotene linoleic acid assay. The antioxidant analysis showed little superoxide dismutase inhibition activity. β-carotene concentrations in aqueous and ethanol extracts were 19.152±0.21mg/g and 22.533±0.82mg/g respectively. High concentrations were observed for vitamins C (79.20±2.56 mg/100g) and E (59.31±2.84 mg/100g). These investigations have revealed the antioxidant potentials of Ginkgo biloba leaves grown in Nigeria. The findings therefore, will be useful for medical practice in Nigeria and beyond for maintenance of individuals’ good health and management of many diseases caused by oxidative stress.
Keywords
Ginkgo biloba, Antioxidants, Oxidative Stress, β-carotene, Free Radicals, Scavenging Activity
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