[01] A. S. M. Ashab Uddin, Training, Planning and Technology Testing Division, Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka, Bangladesh. [02] Md. Amirul Hasan, Training, Planning and Technology Testing Division, Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka, Bangladesh. [03] Md. Ershaduzzaman, Socio-economic Research Division, Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka, Bangladesh. [04] Shamim Ahmed, Training, Planning and Technology Testing Division, Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka, Bangladesh. [05] Md Zulfekar Ali, Animal Health Research Division, Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka, Bangladesh.

Escherichia coli and Shigella spp. isolated from fecal samples of tiger and lion collected from Dhaka Zoo and samples were identified in the Bacteriology Laboratory of Bangladesh Livestock Research Institute, Savar, Dhaka during the period from July to December 2014. A total of 40 samples, 18 from Tiger and 22 from Lion fecal samples were collected aseptically and subjected to primary isolation by propagating in nutrient broth followed by culture on different agar media and both. Gram’s staining and hanging drop techniques were also performed. Biochemical properties of the isolates were studied. Author found that 65% samples were E. coli positive. On the other hand 35% samples were revealed as Shigella containing samples. All the E. coli isolates were found to produce bright pink colonies on MacConkey agar, yellowish green colonies surrounded by an intense yellow green zone on Brilliant Green (BG) agar and characteristic metallic sheen colonies on the Eosine Methyline Blue (EMB) agar. In Gram’s staining technique, all the isolates were pink colored, indicating Gram negative small bacilli while in the hanging drop technique the organisms were motile. All the E. coli isolates fermented dextrose, maltose, lactose, sucrose and mannitol with the production of both acid and gas. The results of catalase, MR and Indole tests with E. coli isolates were positive but VP test was negative. All the isolated Shigella spp. produced colorless colonies on MacConkey agar, SS agar and EMB agar. During biochemical test all the Shigella isolates ferment dextrose and lactose quickly and sucrose slowly by produced only acid and gas. In Gram’s staining showed pink color medium size, rod shaped organism and non motile in hanging drop slide. In antibiotic sensitivity test, most of the E. coli isolates were sensitive to Ciprofloxacin (CIP), Ceftriaxone (CRO), Azithromycin (AZM) and Cefotaxime (CTX) and resistant to Oxytetracyclin (OT), Erythromycin (E), Amoxicillin/Clavulanic acid (AMC) and Cephradin (CE). Isolated Shigella spp. were revealed sensitive to Ciprofloxacin (CIP), Ceftriaxone (CRO), intermediately sensitive to Amoxicillin/Clavulanic acid (AMC) and resistant to Oxytetracyclin (OT), Erythromycin (E), Azithromycin (AZM) and Cephradin (CE).

Escherichia coli, Shigella spp, Tiger, Lion, Antibiogram, Feces

[01] Ahmed, A. M., Kimi Furuta, Kei Shimomura, Yoshio Kasama and Tadashi Shimamoto. (2006). Genetic characterization of multidrug resistance in Shigella spp. from Japan. J. Med. Microbiol. 55(Pt12): 1685-91. [02] Akond, M. A., Hassan, S. M. R., Alam S. and Shirin, M. (2009). Antibiotic resistance of Escherichia coli isolated from poultry and poultry environment of Bangladesh. American Journal of Environmental Sciences. 5: 47-52. [03] Akter, S. (2012). MS thesis on Characterization of E. coli Isolated From Fecal Samples of Zoo Animals. Roll No. 11 Vet Micro JJ 09M; Registration No. 37991; Session: 2011-2012, Dept. of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensing. [04] Ali, M. Z. and Sultana, S. (2012). Isolation and identification of bacteria from tracheas and lungs of buffaloes in Dinajpur. Stamford Journal of Microbiology. 2(1): 31-33. [05] Ali, M. Z., Rahman, M. M. and Sultana, S. (2015). Seroprevalence of Mycoplasma gallisepticum antibody by ELISA and serum plate agglutination test of laying chicken. Veterinary World. 8(1): 9-14. [06] Ali, M. Z. and Sultana, S. (2015). Determination of humoral immune response in chickens against formalin-inactivated alum precipitated Fowl cholera vaccine. International Journal of Animal Biology. 1(4): 114-117. [07] Ali, M. Z., Sultana, S., Rahman, M. T. and Islam, M. S. (2013). Economics of Fertility Management of Small Holding Dairy Farms in Bangladesh. Iranian Journal of Applied Animal Science. 3(3): 509-512. [08] Bentley, R., Meganathan, R. (1982). Biosynthesis of vitamin K (menaquinone) in Bacteria. Microbiol. Rev.46 (3): 241-80. [09] Beutin, L. Geier, D., Zimmeronann, S., Aleksic, S., Gillespie, H. A. and Whittam, T. S. (1997). Epidemiological relatedness and clonal types of natural populations of Escherichia colis trains producing shiga toxin in separate population of cattle and sheep. Applied and environmental Microbiology,63: 2175-2180. [10] Brown, A. E. (2004). Benson’s Microbiological Applications; Laboratory Manual In General Microbiology. Ninth edition. McGraw Hill Publication. P.252. [11] Buxton, A. and Fraser, G. (1977). Animal Microbiology. Escherichia coli. Vol. 1. Balckwell Scientific Publications, Oxford, London, Edinburg, Melbourne. 94-102. [12] Chris, W. F. (2002). Fact sheet of shigellosis. European Association of Zoo and Wild Life Veterinarian (EAZWV) Transmissible Disease Fact Sheet. Sheet No. 94. [13] Chundawat, R. S., Habib, B., Karanth, U., Kawanishi, K., Ahmad Khan, J., Lynam, T., Miquelle, D., Nyhus, P., Sunarto, S., Tilson, R. and Sonam Wang. (2010). Panthera tigris. International Union for Conservation of Nature (IUCN) Red List of Threatened Species. Version 2011.2. [14] Cociu, M., Wagner, G., Micu, N. E. and Mihaeschu, G.(1973). Adaptational gastro-enteritis in Siberian tiger (Panthera tigris altaica) in the Bucharest Zoo. International zoo yearbook. 14:171. [15] Cowan, S. T. (1985). Cowan and Steel’s manual for identification of medical bacteria. 2nd edn. Cambridge University Press, Cambridge, London. 138-139. [16] Derakhshantar, A., Ghanbarpour, R. (2002). A study on avian cellulitis in broiler chickens. Veterivarski Archiv.72:277-284. [17] Freeman, B. A. (1985). Burrow’s Text Book of Microbiology. 22ndedn. In: WB Saunders Company, Philadelphia, London, Toronto, Mexico city, Rio de Janeiro, Sydney, Tokyo, pp. 464-475. [18] Hasina, B. (2006). Enteropathotypic characterization of Escherichia coli isolated from diarrhoeic calves and their antibiogram study. M. S. Thesis, Department of Microbiology and Hygiene, BAU, Mymensingh, p. 68. [19] Honda, T., Akhtar, Q., Glass, R. I. and Golam Kibriya, A. K. M. (1981). A simple assay to detect Escherichia coli producing heat labile enterotoxins; results of a field study of the Biken test in Bangladesh. Lancet. 2: 609. [20] Hussain, M. M., Glass, R. J. and Khan, M. R. (1982). Antibiotic used in a rural community in Bangladesh. Int. J. Epidemiol. 11: 402-405. [21] Jeyasanta, K. I., Aiyamperumal, V., Patterson, J. (2012). Prevalence of Antibiotic Resistant Escherichia coli in Sea Foods of Tuticorin Coast, Southeastern India. Adv Biol Res. 6(2):70-7. [22] Kloss, H. G. and Lang, E. M. (1976). Handbook of zoo medicine: disease and treatment of wild animals in zoos, game parks, circuses and private collections. Van Nostrand Rheinhold Co. New York. Pp-128-130. [23] Luaibi, O. K. (2013). Isolation of Escherichia coli from young lions, dogs and sheep with determination of antibiotic resistance. l7(7):19-25. [24] Nazir, K. H. M. N. H, Rahman, M. B., Nasiruddin, K. M., Akhtar, F., Khan, M. F. R. and Islam, M. S. (2005). Antibiotic sensitivity of Escherichia coli isolated from water and its relation with plasmid profile analysis. Pakistan Journal of Biological Science.8: 1610-1613. [25] Pulling, F. B. (1976). Possible enterotoxemia in a Bengal tiger. Journal of zoo animal medicine. 7:26-30. [26] Sabapara, R. H., Jani, R. G., Purohit, J. H. and Bhuva, C. N.(2010). Survey Study of Bacterial Flora of Asiatic Lion (Panthera leo persica). Zoos' Print (Web version), XXV (9): 11-12. [27] Sandhu, K. S., Clarke, R. C., MeFadden, K., Brouwer, A., Louie, M., Wilson, J., Lior, H. and Gyles, C. L. (1996). Prevalence of the eaeA gene in verotoxigenic Escherichia coli strains from dairy cattle in southwest Ontario. Epidemiology and Infection.116: 1-7. [28] Sathyanarayana Rao, M.; Ramachandra, R. N.; Raghavan, R.; Seshadri, S. J.; Keshavamurthy, B. S. Colisepticaemia 1983. Tiger paper. 10:22-34. [29] Shahrazad, M., Al-Shadeedi, Zahra, M., Al-Hakak, Adel, S., Mousa and Faris A. Al-Obaidi. (2012). Prevalence of some zoonotic causing bacteria and the bacterial shedding levels in animals of Baghdad zoo, Iraq. Research opinions in animal and veterinary sciences.www.roavs.com. ISSN: 2223-0343. [30] Sharada, R., Krishnappa, G., Raghavan, R., Sreevinas, G. and Upandra, H. A. (1999). Isolation and serotyping of E. coil from different pathological conditions in poultry. Indian Journal of Poultry Science 34: 366-369. [31] Singleton, P. (1999). Bacteria in Biology, Biotechnology and Medicine (5th ed.). Wiley. pp. 444–454. ISBN 0-471-98880-4. [32] Sultana S, Ali, M. Z. and Rahman, M. T. 2012. Effect of different dietary calcium and phosphorus ratio on urinary system and incidence of gout in broiler chicks. J. Innov. Dev. Strategy. 6(2): 19-23. [33] Sultana, S., Rashid, S. M. H., Islam, M. N., Ali, M. H., Islam, M. M. and Azam, M. G. (2015). Pathological Investigation of Avian Aspergillosis in Commercial Broiler Chicken at Chittagong District. International Journal of Innovation and Applied Studies. 10 (1): 366-376. [34] Thomas, C. G. A. (1988). Gram-negative Bacilli. In: Medical Microbiology, 6th ed. Bailliere Tindall. 273-74. [35] Vogt, R. L. and Dippold, L. (2005). Escherichia coli O157:H7 outbreak associated with consumption of ground beef, June–July 2002. Public Health Rep.120(2): 174-8. [36] Zaki, S., Nalini, T. S., Rao, M. S., Keshav amurthy, B. S. (1980). Isolation of Shigella flexneri from two tiger cubs. 49 (7): 288.