[01] Gédéon Ngiala Bongo, Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo; Department of Veterinary Microbiology, Parasitology and Immunology, College of Veterinary and Biomedical Sciences, Sokoine University of Agriculture, Morogoro City, Tanzania. [02] Huruma Nelwike Tuntufye, Department of Veterinary Microbiology, Parasitology and Immunology, College of Veterinary and Biomedical Sciences, Sokoine University of Agriculture, Morogoro City, Tanzania. [03] Joseph Malakalinga, Department of Veterinary Microbiology, Parasitology and Immunology, College of Veterinary and Biomedical Sciences, Sokoine University of Agriculture, Morogoro City, Tanzania. [04] Koto-te-Nyiwa Ngbolua, Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo. [05] Aaron Lelo Pambu, Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo. [06] Tshiama Claudine, Teaching and Administration in Nursing Care, Higher Institute of Medical Techniques, Kinshasa City, Democratic Republic of the Congo. [07] Clément Mutunda Mbadiko, Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo. [08] Gisèle Kafuti Makengo, Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo. [09] Pius Tshimankinda Mpiana, Department of Chemistry, Faculty of Science, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo. [10] Théophile Fundu Mbemba, Department of Biology, Faculty of Science, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo. [11] Rudovick Kazwala, Department of Veterinary Medicine and Public Health, College of Veterinary and Medical Sciences, Sokoine University of Agriculture, Morogoro City, Tanzania.

In recent years, Tuberculosis (TB) has re-emerged as a serious public health problem worldwide. The disease spreads more easily in overcrowded settings and in conditions of malnutrition and poverty. The emergence of multidrug resistant and lengthy therapy reduces the patient compliance which comprises TB control strategies. In the current study, the leaves of Terminalia ivorensis, Carapa procera, Fagara macrophylla, Anacardium occidentale, Ficus spp. and Drepanoalpha® were extracted using petroleum ether, ethyl acetate and methanol in order to assess their anti-mycobacterial activity against Mycobacterium tuberculosis H37Rv and Mycobacterium tuberculosis spp. on Middlebrook 7H10 agar using a qualitative approach where the activity was determined by the presence or the absence of growth on the plate. The phytochemical screening was used for the identification of the major groups of compounds found in the extracts. The methanolic extract displayed a good activity on both strains than the petroleum ether and ethyl acetate. The presence of alkaloids, flavonoids, tannins, saponins, anthocyanins, quinones known to be of medicinal importance points out a possible source for anti-mycobacterial agents to address the problem of multidrug resistance. The in vitro findings of this study provide a partial support for the use of these plants in the management of various infectious diseases as lead to drug discovery and should be reiterated and recommended for a clinical trial using an animal model.

Tuberculosis, Middlebrook 7H10 Agar, Anti-mycobacterial Activity, Phytochemical Screening, Medicinal Plants

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