[01] Ogunsina Olabode Isaiah, Department of Biochemistry, Faculty of Science, Adekunle Ajasin University, Akungba Akoko, Nigeria. [02] Olusola Augustine Olusegun, Department of Biochemistry, Faculty of Science, Adekunle Ajasin University, Akungba Akoko, Nigeria. [03] Otitolaiye Catherine Adesola, Department of Biochemistry, Faculty of Science, Sokoto State University, Sokoto, Nigeria. [04] Ayedogbon Oluremi Samson, Department of Biochemistry, Faculty of Science, Adekunle Ajasin University, Akungba Akoko, Nigeria.

Microbial resistance to currently available antimicrobial medicines continues to be a global problem. There has been a tremendous increase in the hunt for additional antibacterial agents from nature in recent years, with plants becoming the primary focus in most regions of the world due to the huge availability of plants that have not been screened for antimicrobial activity. Antibacterial activities of Lannea acida extract and its primary components are thus investigated in this work. The antimicrobial activity of L. acida and its fractions against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Klebsiella pnuemoniae, and Enterobacter aerogenes, as well as Aspergillus favus, Candida albicans, Candida tropicalis, Rhyzopus stolonifera, and Fusarium solani, was determined using agar well diffusion methods. Phytochemical screening of L. acida showed the presence of alkaloids, saponins, tannins, flavonoids and terpenoids. The mean zones of growth inhibition for L. acida and fractions were in the range of 26.33±0.19 to 11.33±0.19mm and 25.33±0.50 to 10± 0.57 while Erythromycin 15µg is 37.66±0.50 to 9.66±0.50 mm respectively. The antifungi activities showed the range of 28.33 ± 0.19 to 9.66±0.39. MIC of both extract and its fraction ranged from 26.33±0.19 to 15.33±0.19 and 22.66±0.38 to 10.33±0.19 respectively. The time-killing kinetics study showed that L. acida and its fractions act as bacteriostatic agents. The observed antimicrobial activity of the extract and its fraction, may be due in large proportion to its major constituent, flavonoids.

Lannea acida, Antibiotic Resistance, Antibacterial, Antifungal

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