[01] Ahmed A. Issa, Biology Department, Faculty of Science, Taif University, Taif, Saudi Arabia; Botany Department, Faculty of Science, Assiut University, Assiut, Egypt. [02] Farag A. Shaieb, Microbiology Department, Faculty of Life Sciences, Omar Almukhtar University, El-Beida, Libya. [03] Reem M. Al-Sefat, Microbiology Department, Faculty of Life Sciences, Omar Almukhtar University, El-Beida, Libya.

This work aimed to cultivate of cloacal algae in wastewater for nutrient and heavy metals removal, resulting biomass can be used for bioactive compounds. Scenedesmus, Ankistrodesmus and Oscillatoria cultivated in Al-Garega wastewater reduced nitrogen to about 90% from the medium. At the same time, phosphorus was also reduced to 91 % form Al-Garega and /or Shahat wastewater types by Scenedesmus, Ankistrodesmus and Lyngbya . Also, Nitzschia, Oscillatoria and Lyngbya reduced TIC about 75% using Shahat wastewater. But ,in case of Euglena and Oscillatoria about 65% reduction using Al-Garega wastes . Oscillatoria and Lyngbya were grown in Al-Garega wastewater were reduced Cd to 88 %, Zn to 78% and Pb to 75% from the wastes. Using the same algae in Shahat waste water, the maximum Cd reduction (72%) was recorded by Scenedesmus and Euglena, while, 80 % reduction of Pb was recorded by Lyngbya and 87 % of Zn was observed by Ankistrodesmus . Both aqueous and ethanol extracts of most algae exhibited significant activity against Bacillus cereus and Escherichia coli ,while low zone of inhibition was observed against, Staphylococcus aureus and Pseudomonas aeruginosa. The inhibitory effect of algae on bacterial strains calculated by inhibition zone was in the following order : O. rubscens > L .muscula >Mixture > N. Palea > E .gracils > S. Acutus > A .monoraphids.

Cloacal Algae, Nutrient Removal, Antibacterial

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